Up-regulation of gelatinases and tissue type plasminogen activator by root canal sealers in human osteoblastic cells
Up-regulation of gelatinases and tissue type plasminogen activator by root canal sealers in human osteoblastic cells
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DOI：
10.1016/j.joen.2007.12.021
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发表时间：
2008-03-01
影响因子：
4.2
通讯作者：
Chang, Yu-Chao
中科院分区：
文献类型：
--
作者：
Huang, Fu-Mei;Yang, Shun-Fa;Chang, Yu-Chao
组织学研究表明，根管封闭剂可引起轻度至重度的炎症改变。然而，关于根管封闭剂引起炎症反应的确切机制的信息很少。基质金属蛋白酶（MMP）和纤溶酶原激活剂（PA）对细胞外基质的蛋白水解似乎是炎症过程进展的关键起始事件。本研究旨在探讨环氧树脂根管充填剂AH 26、氧化锌丁香油酚根管充填剂AH 26、氧化锌丁香油酚根管充填剂N2和一种糊剂N2对人成骨细胞系U2 OS细胞中基质金属蛋白酶（MMPs）和多胺酸（PAs）表达的影响。明胶和酪蛋白酶谱法测定明胶和酪蛋白的水解活性。结果显示，AH 26、Canals和N2对U2 OS细胞具有浓度依赖性的细胞毒性（P <0.05）。明胶酶谱显示U2 OS细胞分泌MMP-2（72 kd）和MMP-9（92 kd）。U2 OS细胞在根管封闭剂作用下MMP-2、MMP-9表达上调（P <0.05）。酪蛋白酶谱显示一条分子量为70 kd的酪蛋白酶带，表明存在组织型纤溶酶原激活物（t-PA）。t-PA在根管封闭剂组中表达明显上调（P <0.05）。结论：明胶酶和t-PA的激活可能在根管封闭剂引起的根尖周炎的发病机制中起重要作用。
Histologic investigations have demonstrated that root canal sealers can induce mild to severe inflammatory alternations. However, there is little information on the precise mechanisms about root canal sealer-induced inflammatory reaction. The proteolysis of extracellular matrix by matrix metalloproteinases (MMPs) and plasminogen activators (PAs) seems to be a key initiating event for the progression of the inflammatory process. The aim of this study was to investigate the effects of epoxy resin-based root canal sealer AH26 and zinc oxide-eugenol-based root canal sealer Canals and one paste sealer N2 on the expression of MMPs and PAs in human osteoblastic cell line U2OS cells. The levels of gelatinolytic and caseinolytic activities were measured by gelatin and casein zymography. The results showed that AH26, Canals, and N2 were cytotoxic to U2OS cells in a concentration-dependent manner (P < .05). The gelatin zymograms revealed that MMP-2 (72 kd) and MMP-9 (92 kd) were secreted by U2OS cells. The exposure of U2OS cells to root canal sealers resulted in the up-regulation of MMP-2 and MMP-9 expression (P < .05). Casein zymography exhibited a caseinolytic band with a molecular weight of 70 kd, indicative of the presence of tissue type plasminogen activators (t-PA). t-PA was also found to be up-regulated by root canal sealers (P < .05). Taken together, the activation of gelatinases and t-PA might play an important role in the pathogenesis of root canal sealer-induced periapical inflammation.