A MICROPLATE METHOD FOR ISOLATION OF VIRUSES FROM INFANTS AND CHILDREN WITH ACUTE RESPIRATORY-INFECTIONS
A MICROPLATE METHOD FOR ISOLATION OF VIRUSES FROM INFANTS AND CHILDREN WITH ACUTE RESPIRATORY-INFECTIONS
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DOI：
10.1111/j.1348-0421.1987.tb01340.x
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发表时间：
1987-01-01
影响因子：
2.6
通讯作者：
ISHIDA, N
中科院分区：
文献类型：
--
作者：
NUMAZAKI, Y;OSHIMA, T;ISHIDA, N
1984年12月至1986年12月期间，采用微孔板技术从患有急性呼吸道感染的婴儿和儿童身上分离病毒。通过使用两种组织培养微孔板，含有人胚胎成纤维细胞（HEF）、HEp-2、Vero和MDCK细胞的HHVM平板和含有次级猴肾细胞的MK平板，从4，254个咽拭子中的1，061个（24.9%）分离出1，080个野外病毒。在这1，080株分离株中，1，003株（92.9%）在HHVM平板中回收，其余77株（7.1%）在MK平板中回收。使用HHVM平板，在MDCK中培养甲型和B型流感病毒，在HEp-2中培养RS病毒，在Vero中培养副流感病毒和腮腺炎病毒，在HEF和HEp-2中培养腺病毒，在HEF、HEp-2和Vero中培养脊髓灰质炎病毒，在HEp-2和Vero中科萨基B病毒，在HEF中培养鼻病毒和埃可病毒，在HEF和HEp-2中培养单纯疱疹病毒，在HEF中培养巨细胞病毒，但MK对副流感病毒和科萨基B病毒的敏感性高于Vero。微孔板法与普通试管法的病毒分离率无差异。对于常规工作，微孔板中的交叉污染可忽略不计。
Between December 1984 and December 1986, a microplate technique was adopted for isolation of viruses from infants and children with acute respiratory infections. By using two kinds of tissue culture microplates, i.e., the HHVM plate, containing human embyronic fibroblast (HEF), HEp-2, Vero and MDCK cells, and the MK plate which contains secondary monkey kidney cells, 1,080 field viruses were isolated from 1,061 (24.9%) out of 4,254 throat swabs. Of these 1,080 isolates, 1,003 (92.9%) were recovered in the HHVM plates and the remaining 77 (7.1%) in the MK plates. With the HHVM plate, influenza A and B viruses were cultivated in MDCK, RS virus in HEp-2, parainfluenza and mumps viruses in Vero, adenoviruses in both HEF and HEp-2, polioviruses in HEF, HEp-2 and Vero, coxsackie B viruses in both HEp-2 and Vero, rhino and echo viruses in HEF, herpes simplex virus in both HEF and HEp-2, and cytomegalovirus in HEF, although MK were more sensitive than Vero to parainfluenza and coxsackie B viruses. There was no difference in the rate of isolation of viruses between the microplate and ordinary tube methods. Cross contamination in the microplates was negligible for routine work.