ESTROGEN INDUCTION OF CYTOCHROME-C-OXIDASE SUBUNIT-III IN RAT HIPPOCAMPUS
ESTROGEN INDUCTION OF CYTOCHROME-C-OXIDASE SUBUNIT-III IN RAT HIPPOCAMPUS
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DOI：
10.1111/j.1471-4159.1992.tb10070.x
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发表时间：
1992-05-01
影响因子：
4.7
通讯作者：
MAGGI, A
中科院分区：
文献类型：
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作者：
BETTINI, E;MAGGI, A
从雌激素刺激的卵巢切除雌性大鼠海马mRNA制备的cDNA文库的差异筛选导致识别单个雌激素诱导的克隆。序列分析鉴定该cDNA为编码酶细胞色素c氧化酶亚基III的基因。细胞色素c氧化酶亚基III的mRNA水平显着增加，早在3小时后，单剂量的激素管理。这种效应在海马体和下丘脑中可见，但在其他大脑区域则没有。由于细胞色素c氧化酶的亚基III是线粒体来源的，参与雌激素效应的机制仍然未知。细胞色素c氧化酶的活性也可以诱导雌激素在海马中的观察表明，这种诱导可能是继发于细胞色素c氧化酶的其他亚基的表达增加或神经元活性的普遍增加。
Differential screening of a cDNA library prepared from mRNA of the hippocampus of estrogen-stimulated ovariectomized female rats led to the identification of a single estrogen-induced clone. Analysis of the sequence identified this cDNA as the gene coding for subunit III of the enzyme cytochrome c oxidase. Cytochrome c oxidase subunit III mRNA levels significantly increased as early as 3 h following the administration of a single dose of hormone. This effect was visible in the hippocampus and in the hypothalamus, but not in the other brain areas examined. Because subunit III of the cytochrome c oxidase is of mitochondrial origin, the mechanism involved in the estrogenic effect is still unknown. The observation that the activity of cytochrome c oxidase can also be induced by estrogens in the hippocampus indicates that this induction may be secondary to the increased expression of the other subunits of cytochrome c oxidase or to the general increase of neuronal activity.