The feed gas composition determines the degree of physical plasma-induced platelet activation for blood coagulation
The feed gas composition determines the degree of physical plasma-induced platelet activation for blood coagulation
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DOI：
10.1088/1361-6595/aaaf0e
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发表时间：
2018-03-01
影响因子：
3.8
通讯作者：
van der Linde, Julia
中科院分区：
文献类型：
--
作者：
Bekeschus, Sander;Brueggemeier, Janik;van der Linde, Julia
长期以来，低温大气(物理)血浆一直被认为是一种有用的血液凝固工具。然而，这种方法的临床适用性还没有得到充分的解决。我们此前已经证明，临床接受的大气压Ar等离子射流(kINPen(R)MED)能够以与黄金标准电灼术类似的性能凝固小鼠的肝脏伤口。我们可以证明，血浆介导的凝血依赖于血小板的激活。在目前的工作中，我们通过研究KINPen(R)在体外抗凝的人供体血液中介导的血小板激活来扩展这一点。我们重点建立了高通量、多参数的血小板激活分析方法，并进行了Ar进气参数的研究，结果如下：(I)在肝素化但在EDTA抗凝的血液中，血浆激活的血小板；(Ii)血浆减少了血小板总数，但增加了微粒数量；(Iii)血浆增加了血小板表面几个活化标志物(CD62P、CD63、CD69和CD41/61)的表达；(Iv)在血小板激活中，湿法和干法Ar等离子体优于含氧和/或氮的进气混合气；(V)血浆介导的血小板激活伴随着血小板聚集。血小板聚集是形成血栓的必要条件。这些发现对于进一步阐明冷物理等离子体介导的血液凝固的分子细节和临床可行性具有重要意义。
Cold atmospheric (physical) plasma has long been suggested to be a useful tool for blood coagulation. However, the clinical applicability of this approach has not been addressed sufficiently. We have previously demonstrated the ability of a clinically accepted atmospheric pressure argon plasma jet (kINPen (R) MED) to coagulate liver incisions in mice with similar performance compared to the gold standard electrocauterization. We could show that plasma-mediated blood coagulation was dependent on platelet activation. In the present work, we extended on this by investigating kINPen (R)-mediated platelet activation in anticoagulated human donor blood ex vivo. With focus on establishing high-throughput, multi-parametric platelet activation assays and performing argon feed gas parameter studies we achieved the following results: (i) plasma activated platelets in heparinized but not in EDTA-anticoagulated blood; (ii) plasma decreased total platelet counts but increased numbers of microparticles; (iii) plasma elevated the expression of several surface activation markers on platelets (CD62P, CD63, CD69, and CD41/61); (iv) in platelet activation, wet and dry argon plasma outperformed feed gas admixtures with oxygen and/or nitrogen; (v) plasma-mediated platelet activation was accompanied by platelet aggregation. Platelet aggregation is a necessary requirement for blood clot formation. These findings are important to further elucidate molecular details and clinical feasibility of cold physical plasma-mediated blood coagulation.