AnInR/mir-9a/NlUbxregulatory cascade regulates wing diphenism in brown planthoppers
AnInR/mir-9a/NlUbxregulatory cascade regulates wing diphenism in brown planthoppers
复制标题
InR / mirâ9a / NlUbx 级联调控褐飞虱翅膀双显现象
DOI：
10.1111/1744-7917.12872
复制
发表时间：
2020-10-08
期刊：
影响因子：
4
通讯作者：
Hua, Hong-Xia
中科院分区：
文献类型：
--
作者：
Li, Xiang;Zhao, Mu-Hua;Hua, Hong-Xia
翅的多态性对某些昆虫物种的生态成功起着重要作用。例如，褐飞虱（BPH）褐飞虱（Nilaparvata lugens）是亚洲最具破坏性的水稻害虫之一，它可以发育成高度移动的长翅或高度繁殖的短翅成虫变体。最近的一项研究报告了一个高度挑衅性的结果，即Hox基因Ultrabithorax（Ubx）在BPH前翅中表达，并表明这种翅膀发育基因在发育成长翅和短翅变体的鸟类中差异表达。在此，我们发现Ubx可能是amir-9的靶点，并使用双荧光素酶报告基因检测和注射microRNA（miRNA）模拟物和抑制剂来证实mir-9a和NlUbx之间的相互作用。我们检测了miR-9a和NlUbx在大肠杆菌中的表达谱，发现这两种生物分子的表达呈负相关。通过在不同营养状态的宿主水稻上饲养BPH，我们能够描述胰岛素受体基因mir-9a和NlUbx之间调节BPH翅长的级联反应。当寄主质量较低时，翅长中NlInR 1的表达量增加，NlInR 2的表达量减少，这导致了更高的ermir-9a水平，进而降低了NlUbx的转录水平，最终导致翅长的增加。除了扩展我们对宿主植物状态和调节多态性的遗传事件之间相互作用的理解之外，我们还展示了控制BPH前翅中Ubx表达的上游信号和基于miRNA的调控机制。
Wing polymorphism significantly contributes to the ecological success of some insect species. For example, the brown planthopper (BPH)Nilaparvata lugens, which is one of the most destructive rice pests in Asia, can develop into either highly mobile long-winged or highly fecund short-winged adult morphs. A recent study reported a highly provocative result that theHoxgeneUltrabithorax(Ubx) is expressed in BPH forewings and showed that this wing development gene is differentially expressed in nymphs that develop into long-winged versus short-winged morphs. Here, we found thatUbxmay be amir-9atarget, and used dual luciferase reporter assays and injected micro RNA (miRNA) mimics and inhibitors to confirm the interactions betweenmir-9aandNlUbx. We measured themir-9aandNlUbxexpression profiles in nymphs and found that the expression of these two biomolecules was negatively correlated. By rearing BPH nymphs on host rice plants with different nutritional status, we were able to characterize a regulatory cascade between insulin receptor genes,mir-9a, andNlUbxthat regulate wing length in BPHs. When host quality was low,NlInR1expression in the nymph terga increased andNlInR2expression decreased; this led to a highermir-9alevel, which in turn reduced theNlUbxtranscript level and ultimately resulted in longer wing lengths. Beyond extending our understanding of the interplay between host plant status and genetic events that modulate polymorphism, we demonstrated both the upstream signal and miRNA-based regulatory mechanism that controlUbxexpression in BPH forewings.