The Origin of Plasma-Derived Bacterial Extracellular Vesicles in Healthy Individuals and Patients with Inflammatory Bowel Disease: A Pilot Study.
The Origin of Plasma-Derived Bacterial Extracellular Vesicles in Healthy Individuals and Patients with Inflammatory Bowel Disease: A Pilot Study.
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健康个体和炎症性肠病患者血浆来源的细菌胞外囊泡的起源：一项初步研究。
DOI：
10.3390/genes12101636
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发表时间：
2021-10-18
期刊：
影响因子：
3.5
通讯作者：
Carding SR
中科院分区：
文献类型：
--
作者：
Jones E;Stentz R;Telatin A;Savva GM;Booth C;Baker D;Rudder S;Knight SC;Noble A;Carding SR
胃肠道是肠道微生物群的栖息地，其结构改变（生态失调）与肠道通透性增加（“漏肠”）有关，使腔内抗原和细菌产物（如纳米细菌细胞外囊泡（BEVs））能够进入循环系统。血液来源的bev含有各种货物，可能是诊断和监测疾病状态和炎症性肠病（IBD）等疾病复发的有用生物标志物。为了推进这一概念，我们开发了一种快速、经济高效的方案来分离bev相关DNA，并使用16S rRNA基因测序来鉴定健康个体和克罗恩病和溃疡性结肠炎患者血液微生物组的细菌来源。16S rRNA基因测序成功鉴定了血浆源性BEV DNA的来源。分析显示，IBD组、健康对照组和方案对照组的血液微生物群丰富度、多样性或组成没有显著差异，这突出了低生物量研究中的“kit-ome”污染问题。我们的初步研究为开展更大规模的研究提供了基础，以确定血液微生物群分析作为IBD诊断辅助手段的潜在用途。
The gastrointestinal tract harbors the gut microbiota, structural alterations of which (dysbiosis) are linked with an increase in gut permeability (“leaky gut”), enabling luminal antigens and bacterial products such as nanosized bacterial extracellular vesicles (BEVs) to access the circulatory system. Blood-derived BEVs contain various cargoes and may be useful biomarkers for diagnosis and monitoring of disease status and relapse in conditions such as inflammatory bowel disease (IBD). To progress this concept, we developed a rapid, cost-effective protocol to isolate BEV-associated DNA and used 16S rRNA gene sequencing to identify bacterial origins of the blood microbiome of healthy individuals and patients with Crohn’s disease and ulcerative colitis. The 16S rRNA gene sequencing successfully identified the origin of plasma-derived BEV DNA. The analysis showed that the blood microbiota richness, diversity, or composition in IBD, healthy control, and protocol control groups were not significantly distinct, highlighting the issue of ‘kit-ome’ contamination in low-biomass studies. Our pilot study provides the basis for undertaking larger studies to determine the potential use of blood microbiota profiling as a diagnostic aid in IBD.
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影响因子：
15.5
作者：
Durant, Lydia;Stentz, Regis;Knight, Stella C.
通讯作者：
Knight, Stella C.
影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
4.6
作者：
Brennan, K.;Martin, K.;Mc Gee, M. M.
通讯作者：
Mc Gee, M. M.
影响因子：
2.1
作者：
Grahn, N;Olofsson, M;Jonasson, J
通讯作者：
Jonasson, J