Regulation of flowering time by the histone deacetylase HDA5 in Arabidopsis
Regulation of flowering time by the histone deacetylase HDA5 in Arabidopsis
复制标题
组蛋白脱乙酰酶 HDA5 调控拟南芥开花时间
DOI：
10.1111/tpj.12868
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发表时间：
2015-06-01
期刊：
影响因子：
7.2
通讯作者：
Wu, Keqiang
中科院分区：
文献类型：
--
作者：
Luo, Ming;Tai, Ready;Wu, Keqiang
组蛋白乙酰化水平受组蛋白乙酰转移酶和组蛋白去乙酰基酶的调控，在植物基因表达调控中发挥着重要的作用，但研究较少。为了研究拟南芥RPD3/HDA1家族组蛋白去乙酰基酶HDA5的特性，我们提出了HDA5具有去乙酰基酶活性的证据。HDA5表达缺失的突变体表现为晚花表型。在hda5突变体中，开花抑制基因FLC和MAF1的表达上调。此外，hda5-1突变体的FLC和MAF1染色质上的组蛋白H3乙酰化和H3K4三甲基化等基因激活标记增加。染色质免疫沉淀分析表明，HDA5与FLC和MAF1的染色质结合。双分子荧光互补实验和免疫共沉淀实验表明，HDA5与FVE、FLD和HDA6相互作用，表明这些蛋白质以蛋白质复合体的形式存在，参与了开花时间的调控。通过RNA-seq比较hda5和hda6突变体的基因表达谱发现，hda5和hda6在多个发育过程和途径中共同调节基因的表达。意义陈述我们发现组蛋白脱乙酰基酶5(HDA5)和hda6与FVE和FLD形成HDAC阻遏复合体，参与拟南芥开花时间的调控。
The acetylation level of histones on lysine residues regulated by histone acetyltransferases and histone deacetylases plays an important but under-studied role in the control of gene expression in plants. With the aim of characterizing the Arabidopsis RPD3/HDA1 family histone deacetylase HDA5, we present evidence showing that HDA5 displays deacetylase activity. Mutants defective in the expression of HDA5 displayed a late-flowering phenotype. Expression of the flowering repressor genes FLC and MAF1 was up-regulated in hda5 mutants. Furthermore, the gene activation markers, histone H3 acetylation and H3K4 trimethylation on FLC and MAF1 chromatin were increased in hda5-1 mutants. Chromatin immunoprecipitation analysis showed that HDA5 binds to the chromatin of FLC and MAF1. Bimolecular fluorescence complementation assays and co-immunoprecipitation assays showed that HDA5 interacts with FVE, FLD and HDA6, indicating that these proteins are present in a protein complex involved in the regulation of flowering time. Comparing gene expression profiles of hda5 and hda6 mutants by RNA-seq revealed that HDA5 and HDA6 co-regulate gene expression in multiple development processes and pathways.Significance Statement We found that HISTONE DEACETYLASE 5 (HDA5) and HDA6 form a HDAC repression complex with FVE and FLD involved in the regulation of flowering time in Arabidopsis.