SARCOMA-VIRUSES CARRYING RAS ONCOGENES INDUCE DIFFERENTIATION-ASSOCIATED PROPERTIES IN A NEURONAL CELL-LINE
SARCOMA-VIRUSES CARRYING RAS ONCOGENES INDUCE DIFFERENTIATION-ASSOCIATED PROPERTIES IN A NEURONAL CELL-LINE
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DOI：
10.1038/318073a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
IKAWA, Y
中科院分区：
文献类型：
--
作者：
NODA, M;KO, M;IKAWA, Y
柯尔斯滕病毒(Ki-MSV)或哈维小鼠肉瘤病毒(Ha-MSV)在体外对不同类型的细胞具有促生长和/或分化抑制作用。在这里，我们报告了这些病毒对大鼠嗜铬细胞瘤细胞系PC12的意外影响。PC12细胞在生长介质中无限增殖，已知通过停止细胞分裂和表达一些类似于分化的交感神经元的特性来对神经生长因子(NGF)做出反应。我们已经发现Ki和Ha-MSV模拟了PC12细胞中NGF的部分(如果不是全部)活性，并且有证据表明病毒癌基因v-Ki-ras和v-Ha-ras与这种现象有关。该系统对研究v-ras基因的作用机制以及神经细胞生长分化的调控机制有一定的参考价值。
The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha-MSV) on various types of cells in vitro are well documented. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones. We have found that Ki and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.