Enzyme evolution and the development of metabolic pathways
Enzyme evolution and the development of metabolic pathways
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酶的进化和代谢途径的发展
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Antonio Reyes
中科院分区：
文献类型：
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作者：
J. Peretó;R. Fani;J. Leguina;Antonio Reyes
当阿道夫·冯·拜耳得知爱德华·布赫纳因发现酶而获得1907年诺贝尔化学奖时，他评论道：“这将为布赫纳带来名声，尽管他没有化学天赋”(Bloch，1994)。然而，尽管爱德华·布克纳第一次观察到无细胞发酵是在他哥哥汉斯的实验室里进行的一次获得免疫活性细菌蛋白质的试运行中进行的，但它们最终将成为新的生物化学科学的基石之一。尽管那时对可溶性酶的研究已经是一个发达的研究领域，但它们被认为在生物学上远不如原生质本身重要，许多人仍然坚持T.H.赫胥黎的断言，即“原生质，无论是简单的还是有核的，都是所有生命的形式基础”(Huxley，1870)。人们认识到无细胞发酵是由于酶的活性，这一认识迅速挑战了原生质生物学的观点，并导致了一种新的以酶为中心的机械论生命理论(Kohler，1971,1972)。尽管关于原生质的实际性质存在不同的观点，有时甚至是相反的观点(Ling，1984；Welch，1995)，但这项研究
When Adolph von Bayer learned that Eduard Buchner had been awarded the 1907 Nobel Prize in Chemistry for his discovery of zymase, he remarked that “this will bring Buchner fame, even though he has no chemical talent” (Bloch, 1994). And yet, although Eduard Buchner’s first observations of cell-free fermentation had taken place during a trial run to obtain immunologically active bacterial proteins in his brother Hans’ laboratory, they would eventually become one of the cornerstones of the new science of biochemistry. Although by then the study of soluble enzymes was already a well-developed area of research, they were considered as biologically much less important than protoplasm itself, and many still adhered to T. H. Huxley’s assertion that “protoplasm, simple or nucleated, is the formal basis of all life” (Huxley, 1870). The recognition that cell-free fermentation was due to enzymatic activity rapidly challenged the views of protoplasmic biology, and led to a new mechanistic enzyme-centred theory of life (Kohler, 1971, 1972). Although different and sometimes opposing ideas on the actual nature of the protoplasm coexisted (Ling, 1984; Welch, 1995), the study
具有氨基咪唑核糖核苷酸合成酶、甘氨酰胺核糖核苷酸合成酶和甘氨酰胺核糖核苷酸转化酶活性的多功能蛋白质的分离：氨基咪唑核糖核苷酸合成酶的表征。
影响因子：
2.9
作者：
Schrimsher,JL;Schendel,FJ;Stubbe,J
通讯作者：
Stubbe,J