Characterization of tests of functional recovery after median and ulnar nerve injury and repair in the rat forelimb
Characterization of tests of functional recovery after median and ulnar nerve injury and repair in the rat forelimb
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DOI：
10.1111/j.1529-8027.2007.00113.x
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发表时间：
2007-03-01
影响因子：
3.8
通讯作者：
Fawcett, James W.
中科院分区：
文献类型：
--
作者：
Galtrey, Clare M.;Fawcett, James W.
大多数人类周围神经损伤发生在上肢，但大多数大鼠研究是在后肢进行的。上肢和下肢在灵活性和由脊髓上系统控制方面不同，因此上肢模型更好地代表了人类常见的损伤形式。本研究的目的是进一步发展一种涉及正中神经和尺神经损伤的大鼠模型。为了在神经修复后产生不同程度的轴突错误方向，我们研究了神经挤压、两条神经的切割和修复以及交叉切割和修复。使用一系列运动和感觉测试进行功能恢复的评估：楼梯测试，其评估熟练的前爪伸展;握力计，其评估握力;爪印分析，其评估脚趾伸展和爪印长度;水平梯，其评估熟练运动期间的前爪放置;改良的Randall-Selitto装置和电子von Frey探针，其评估精细触摸;和冷探头，用于评估温度感觉。所有测试均显示神经损伤后前爪功能的缺陷，除了打印长度和改良的Randall-Selitto装置。观察15周内功能恢复的时间进程。最终达到的功能恢复程度与轴突再生的错误方向有关。最清楚地揭示轴突错误方向对功能的影响的测试是熟练的爪子到达和握力测试。我们开发的损伤模型和功能测试将有助于测试治疗策略，用于治疗人类周围神经损伤后轴突再生不准确的后果。
The majority of human peripheral nerve injuries occur in the upper limb but the majority of studies in the rat are performed in the hindlimb. The upper and lower limbs differ in dexterity and control by supraspinal systems, so an upper limb model is a better representation of the common form of human injury. The purpose of this study was to further develop a rat model involving lesions of the median and ulnar nerves. To produce different degrees of misdirection of axons following nerve repair, we studied nerve crush, cut and repair of the two nerves, and cut and repair with crossover. Assessment of functional recovery was performed using a battery of motor and sensory tests: the staircase test, which assesses skilled forepaw reaching; grip strength meter, which assesses grip strength; pawprint analysis, which assesses toe spread and print length; horizontal ladder, which assesses forepaw placement during skilled locomotion; modified Randall-Selitto device and electronic von Frey probes, which assess fine touch; and cold probes, which assess temperature sensation. All tests revealed deficits in forepaw function after nerve injury except the print length and modified Randall-Selitto device. The time course of functional recovery was observed over 15 weeks. The final degree of functional recovery achieved was related to the misdirection of axon regeneration. The tests that most clearly revealed the effects of axon misdirection on function were the skilled paw reaching and grip strength tests. The lesion model and functional tests that we have developed will be useful in testing therapeutic strategies for treating the consequences of inaccurate axon regeneration following peripheral nerve injury in humans.