The calcium-dependent protein kinase (CDPK) and CDPK-related kinase gene families in Hevea brasiliensis-comparison with five other plant species in structure, evolution, and expression
The calcium-dependent protein kinase (CDPK) and CDPK-related kinase gene families in Hevea brasiliensis-comparison with five other plant species in structure, evolution, and expression
复制标题
巴西橡胶树钙依赖性蛋白激酶（CDPK）和CDPK相关激酶基因家族——与其他五种植物的结构、进化和表达比较
DOI：
10.1002/22115463.12163
复制
发表时间：
2017
期刊：
影响因子：
2.6
通讯作者：
Tang Chao-Rong
中科院分区：
文献类型：
--
作者：
Xiao Xiao-Hu;Yang Meng;Sui Jin-Lei;Qi Ji-Yan;Fang Yong-Jun;Hu Song-Nian;Tang Chao-Rong
钙依赖性蛋白激酶（CDPKs或CPKs）在植物的各种生理过程中发挥重要作用，包括生长发育，胁迫反应和激素信号传导。虽然CDPK基因家族已经在几种模式植物中得到了表征，但对Hevea brasiliensis（帕拉橡胶树）中的该基因家族知之甚少。在这里，我们描述了整个H的特征。巴西人CDPK和CDPK相关激酶（CRK）基因家族，包括30个CDPK基因（HbCPK 1至30）和9个CRK基因（HbCRK 1至9）。对这些CDPK和CRK基因的结构和遗传学分析表明，它们在H染色体上具有进化保守性。brasiliensisand other plant植物species种类.通过Solexa测序研究了HbCPK和HbCRK基因在一系列实验条件（不同组织、叶片发育阶段、乙烯处理和各种非生物胁迫）下的表达。这些结果表明，HbCPK和HbCRK基因是影响红球藻生长发育和应激反应的重要基因。巴西的。对CDPK和CRK基因家族的平行研究也扩展到其他五种植物（拟南芥、水稻、大果杨、木薯和蓖麻）。来自不同植物物种的CDPK和CRK基因表现出相似的表达模式，倾向于聚集在一起，这表明高等植物中基因结构和表达行为的协同进化。这些结果为进一步研究这些基因家族在H中的功能奠定了基础。brasiliensisas以及整个植物王国。
Calcium‐dependent protein kinases (CDPKs or CPKs) play important roles in various physiological processes of plants, including growth and development, stress responses and hormone signaling. Although the CDPK gene family has been characterized in several model plants, little is known about this gene family inHevea brasiliensis(the Para rubber tree). Here, we characterize the entireH. brasiliensisCDPK and CDPK‐related kinase (CRK) gene families comprising 30CDPKgenes (HbCPK1to30) and nineCRKgenes (HbCRK1to9). Structure and phylogeny analyses of theseCDPKandCRKgenes demonstrate evolutionary conservation in these gene families acrossH. brasiliensisand other plant species. The expression ofHbCPKandHbCRKgenes was investigated via Solexa sequencing in a range of experimental conditions (different tissues, phases of leaf development, ethylene treatment, and various abiotic stresses). The results suggest thatHbCPKandHbCRKgenes are important components in growth, development, and stress responses ofH. brasiliensis. Parallel studies on theCDPKandCRKgene families were also extended to five other plant species (Arabidopsis thaliana,Oryza sativa,Populus trichocarpa,Manihot esculenta,andRicinus communis). TheCDPKandCRKgenes from different plant species that exhibit similar expression patterns tend to cluster together, suggesting a coevolution of gene structure and expression behavior in higher plants. The results serve as a foundation to further functional studies of these gene families inH. brasiliensisas well as in the whole plant kingdom.