A ketogenic diet reduces metabolic syndrome-induced allodynia and promotes peripheral nerve growth in mice
A ketogenic diet reduces metabolic syndrome-induced allodynia and promotes peripheral nerve growth in mice
复制标题
DOI：
10.1016/j.expneurol.2018.05.011
复制
发表时间：
2018-08-01
影响因子：
5.3
通讯作者：
Wright, Douglas E.
中科院分区：
文献类型：
--
作者：
Cooper, Michael A.;Menta, Blaise W.;Wright, Douglas E.
目前的实验研究了生酮饮食是否会影响与肥胖和糖尿病前期相关的神经病变。将接受生酮饮食挑战的小鼠与喂食高脂肪饮食或高脂肪饮食加运动的小鼠进行比较。此外，还进行了 8 周高脂饮食后转为生酮饮食的干预措施，以比较控制饮食、运动或生酮饮食如何影响代谢综合征引起的神经并发症。当接受生酮饮食挑战时，与高脂肪喂养的小鼠相比，小鼠的体重和脂肪量有所减少，并且与运动的高脂肪喂养的小鼠相似。高脂肪喂养、运动和生酮喂养的小鼠血糖轻度升高；相反，生酮饮食喂养的小鼠的血清胰岛素水平降低是独一无二的。与高脂肪饮食喂养的小鼠相反，生酮饮食喂养的小鼠从未出现机械性异常性疼痛。与所有其他组相比，生酮饮食喂养的小鼠表皮轴突密度也有所增加。当生酮饮食作为干预措施时，生酮饮食无法逆转高脂饮食引起的代谢变化，但能够显着逆转高脂饮食引起的机械异常性疼痛。作为干预措施，生酮饮食还增加了表皮轴突密度。体外研究表明，生酮饮食喂养的小鼠的感觉神经元和在培养基中给予酮体的正常饮食喂养的小鼠的神经元中，神经突生长增加。这些结果表明生酮饮食可以预防糖尿病前期的某些并发症，并对周围轴突和感觉功能障碍提供显着益处。
Current experiments investigated whether a ketogenic diet impacts neuropathy associated with obesity and prediabetes. Mice challenged with a ketogenic diet were compared to mice fed a high-fat diet or a high-fat diet plus exercise. Additionally, an intervention switching to a ketogenic diet following 8 weeks of high-fat diet was performed to compare how a control diet, exercise, or a ketogenic diet affects metabolic syndrome-induced neural complications. When challenged with a ketogenic diet, mice had reduced bodyweight and fat mass compared to high-fat-fed mice, and were similar to exercised, high-fat-fed mice. High-fat-fed, exercised and ketogenic-fed mice had mildly elevated blood glucose; conversely, ketogenic diet-fed mice were unique in having reduced serum insulin levels. Ketogenic diet-fed mice never developed mechanical allodynia contrary to mice fed a high-fat diet. Ketogenic diet fed mice also had increased epidermal axon density compared all other groups. When a ketogenic diet was used as an intervention, a ketogenic diet was unable to reverse high-fat fed induced metabolic changes but was able to significantly reverse a high-fat diet-induced mechanical allodynia. As an intervention, a ketogenic diet also increased epidermal axon density. In vitro studies revealed increased neurite outgrowth in sensory neurons from mice fed a ketogenic diet and in neurons from normal diet-fed mice given ketone bodies in the culture medium. These results suggest a ketogenic diet can prevent certain complications of prediabetes and provides significant benefits to peripheral axons and sensory dysfunction.