DETECTION AND CHARACTERIZATION OF HIGH-AFFINITY PLASMA-MEMBRANE RECEPTORS FOR HUMAN INTERLEUKIN-1
DETECTION AND CHARACTERIZATION OF HIGH-AFFINITY PLASMA-MEMBRANE RECEPTORS FOR HUMAN INTERLEUKIN-1
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DOI：
10.1084/jem.162.2.501
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发表时间：
1985-01-01
影响因子：
15.3
通讯作者：
URDAL, DL
中科院分区：
文献类型：
--
作者：
DOWER, SK;KRONHEIM, SR;URDAL, DL
白细胞介素1 （IL-1）是一种多肽激素，作为炎症的中心介质。由于IL-1的作用可能是由特定的细胞表面受体介导的，因此表征了这种激素与细胞的结合。纯化的人IL-1用125I标记为高比活性，使用Bolton-Hunter试剂。标记的蛋白特异性结合LBRM-33-1A5（一种小鼠T淋巴瘤系，先前显示在植物血凝素和IL-1的反应中产生IL-2），与.apprx具有亲和力。0.2 - 2同学们。1010/M，在饱和时，约为1010/M。500个受体/细胞，在8度下的完整细胞。C在叠氮化钠的存在下。未修饰的IL-1对小鼠质膜受体的亲和力为0.9-2倍。1010/M，通过抑制125I-IL-1结合来测定。小鼠受体特异性被证实，在一系列12多肽激素中，只有IL-1抑制125I-IL-1与LBRM-33-1A5细胞的结合。用二价水溶性交联剂处理表面结合的125-I-IL-1，鉴定出与IL-1交联的m79,500膜多肽。研究了多种细胞类型特异性结合125I-IL-1的能力。特异性结合的存在与细胞对IL-1反应的能力相关。多肽激素IL-1的生物学效应是由高亲和质膜受体介导的。这些受体的鉴定将为了解IL-1明显多样的生物活性提供有价值的见解。
Interleukin 1 (IL-1) is a polypeptide hormone that acts as a central mediator of inflammation. Since IL-1 action is presumably mediated by specific cell surface receptor(s), the binding of this hormone to cells was characterized. Purified human IL-1 was labeled to high specific activity with 125I, using Bolton-Hunter reagent. The labeled protein binds specifically to LBRM-33-1A5 (a murine T lymphoma line previously shown to produce IL-2 in response to phytohemagglutinin and IL-1) with an affinity to .apprx. 0.2-2 .times. 1010/M and, at saturation, to .apprx. 500 receptors/cell, on intact cells at 8.degree. C in the presence of sodium azide. The affinity of unmodified IL-1 for the murine plasma membrane receptor is 0.9-2 .times. 1010/M, as measured by the inhibition of 125I-IL-1 binding. The murine receptor specificity was confirmed by demonstrating that, among a series of 12 polypeptide hormones, only IL-1 inhibits 125I-IL-1 binding to LBRM-33-1A5 cells. Treatment of surface-bound 125-I-IL-1 with bivalent water-soluble crosslinkers identified a membrane polypeptide of MW 79,500 to which IL-1 is crosslinked. A variety of cell types were surveyed for the capacity to bind 125I-IL-1 specifically. The presence of specific binding correlates with the capacity of the cells tested to respond to IL-1. The biological effects of the polypeptide hormone IL-1 are mediated by high affinity plasma membrane receptors. The identification of these receptors should provide valuable insight into the apparently diverse biological activities of IL-1.