Clustering of clinical strains of Helicobacter pylori analyzed by two-dimensional gel electrophoresis
Clustering of clinical strains of Helicobacter pylori analyzed by two-dimensional gel electrophoresis
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DOI：
10.1128/cdli.7.2.301-306.2000
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发表时间：
2000-03-01
期刊：
影响因子：
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通讯作者：
Engstrand, L
中科院分区：
文献类型：
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作者：
Enroth, H;Åkerlund, T;Engstrand, L
幽门螺杆菌的菌株变异已经通过多种方法进行了测试，并在不同的患者群体之间进行了比较。本研究的目的是探讨H.幽门螺杆菌表达疾病特异性蛋白质，可以通过二维聚丙烯酰胺凝胶电泳（2-D PAGE）检测。H.分析了从十二指肠溃疡、胃癌和胃炎患者中分离的幽门螺杆菌菌株。菌株之间观察到斑点模式的广泛变化，但树状图分析显示，每个疾病组内的一些菌株聚集在一起。对H. pylori基因组序列。2-D PAGE是研究蛋白质表达和突出H. pylori显示。
Strain variations of Helicobacter pylori have been tested by numerous methods and compared among different patient groups. The aim of this study was to investigate whether H. pylori expresses disease-specific proteins that can be detected by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). H. pylori strains isolated from duodenal ulcer, gastric cancer, and gastritis patients were analyzed. Extensive variation in spot patterns was observed between the strains, but a dendrogram analysis revealed that some strains within each disease group clustered together. Eight proteins were sequenced and found in the H. pylori genome sequence. 2-D PAGE is a useful method for studies of protein expression and for highlighting the extensive strain variation that H. pylori exhibits.