INHIBITION OF ENDOTHELIAL REGENERATION BY TYPE-BETA TRANSFORMING GROWTH-FACTOR FROM PLATELETS
INHIBITION OF ENDOTHELIAL REGENERATION BY TYPE-BETA TRANSFORMING GROWTH-FACTOR FROM PLATELETS
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DOI：
10.1126/science.3461562
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发表时间：
1986-09-05
期刊：
影响因子：
56.9
通讯作者：
SCHWARTZ, SM
中科院分区：
文献类型：
--
作者：
HEIMARK, RL;TWARDZIK, DR;SCHWARTZ, SM
血管壁损伤是血管内皮细胞迁移和复制以及损伤部位血小板释放的信号。添加转化生长因子-β(转化生长因子-β)从生长中的主动脉内皮细胞中提取的纯品可浓度依赖性地抑制[~3H]胸腺嘧啶核苷的掺入。损伤后24小时内，细胞迁移和复制受到抑制，DNA合成也受到一过性抑制。伤后48小时，经转化生长因子β处理的和未处理的培养物显示出相似的反应。流式细胞仪对细胞周期分布的分析表明，在暴露于转化生长因子-β24小时后。细胞进入S时相受阻，G1期细胞比例增加。转化生长因子-β对再生启动的抑制作用。可使平滑肌细胞有时间重新聚集到其他血小板成分损伤的部位。
Damage to the vessel wall is a signal for endothelial migration and replication and for platelet release at the site of injury. Addition of transforming growth factor-beta (TGF-.beta.) purified from platelets to growing aortic endothelial cells inhibited [3H]thymidine incorporation in a concentration-dependent manner. A transient inhibition of DNA synthesis was also observed in response to wounding; cell migration and replication are inhibited during the first 24 hours after wounding. By 48 hours after wounding both TGF-.beta.-treated and -untreated cultures showed similar responses. Flow microfluorimetric analysis of cell cycle distribution indicated that after 24 hours of exposure to TGF-.beta. the cells were blocked from entering S phase, and the fraction of cells in G1 was increased. The inhibition of the initiation of regeneration by TGF-.beta. could allow time for recruitment of smooth muscle cells into the site of injury by other platelet components.