Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid
Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid
复制标题
由 Fmoc-鸟嘌呤缀合肽核酸形成的机械刚性超分子组件
DOI：
10.1038/s41467-019-13250-x
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发表时间：
2019-11-20
影响因子：
16.6
通讯作者：
Gazit, Ehud
中科院分区：
文献类型：
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作者：
Basavalingappa, Vasantha;Bera, Santu;Gazit, Ehud
DNA结构的多样性和复杂性使其成为纳米技术的候选对象，但与聚酰胺分子相比，稳定性和机械刚性不足限制了它们的应用。在这里，我们结合了聚酰胺材料的优点和核酸激发的结构模式，合成了一种具有不同形貌和光致发光性质的机械刚性的荧甲氧甲酰基(FMOC)-鸟氨酸肽核酸(PNA)偶联物。该组装体具有独特的原子结构，一个分子的每个鸟嘌呤头部氢键连接到另一个分子的Fmoc羰基尾部，产生非平面的环状四元组排列。该结构的平均刚性为69.6 ± 6.8 N m−1，杨氏模数为17.8 ± 2.5 GPA，高于以往报道的任何核酸衍生结构。这些数据表明，Fmoc-G-PNA共轭形成的独特的无阳离子篮子可以作为设计基于PNA自组装的纳米技术应用的新材料的一个有吸引力的组成部分。
The variety and complexity of DNA-based structures make them attractive candidates for nanotechnology, yet insufficient stability and mechanical rigidity, compared to polyamide-based molecules, limit their application. Here, we combine the advantages of polyamide materials and the structural patterns inspired by nucleic-acids to generate a mechanically rigid fluorenylmethyloxycarbonyl (Fmoc)-guanine peptide nucleic acid (PNA) conjugate with diverse morphology and photoluminescent properties. The assembly possesses a unique atomic structure, with each guanine head of one molecule hydrogen bonded to the Fmoc carbonyl tail of another molecule, generating a non-planar cyclic quartet arrangement. This structure exhibits an average stiffness of 69.6 ± 6.8 N m−1and Young’s modulus of 17.8 ± 2.5 GPa, higher than any previously reported nucleic acid derived structure. This data suggests that the unique cation-free “basket” formed by the Fmoc-G-PNA conjugate can serve as an attractive component for the design of new materials based on PNA self-assembly for nanotechnology applications.