Designing of Combined Nano and Microfiber Network by Immobilization of Oxidized Cellulose Nanofiber on Polycaprolactone Fibrous Scaffold
Designing of Combined Nano and Microfiber Network by Immobilization of Oxidized Cellulose Nanofiber on Polycaprolactone Fibrous Scaffold
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DOI：
10.1166/jbn.2016.2308
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发表时间：
2016-10-01
影响因子：
2.9
通讯作者：
Lee, Byong-Taek
中科院分区：
文献类型：
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作者：
Amirian, Jhaleh;Lee, Sun-Young;Lee, Byong-Taek
在这项研究中，氧化纤维素纤维（OCNF）网络的固定在聚己内酯（PCL）的纤维支架上进行的氨解过程通过静电纺丝和层层（LBL）技术。通过场发射扫描电镜（FE-SEM）观察了支架的形貌，结果表明，OCNF固定在PCL上后，形成了独特的纳米网状结构。采用X射线光电子能谱（XPS）、傅里叶变换光谱（FT-IR）、热重分析（TGA）、水接触角测量和BSA吸附性能等方法对样品的理化性质进行了研究。此外，通过在纤维支架上接种大鼠骨髓干细胞（RBMSCs）来评估细胞附着、细胞活力和增殖，检查了对经OCNF修饰和未经OCNF修饰的PCL支架的细胞反应。因此，本研究的重点是制备和表征的膜和管状支架具有独特的纳米结构，这是一个很好的候选人用作血管支架移植物。
In this study, the immobilization of oxidized cellulose nanofiber (OCNF) network on the polycaprolactone (PCL) fibrous scaffold was performed by the aminolysis procedure through electrospinning and layer by layer (LBL) techniques. The morphology of the fibrous scaffold was examined by field emission scanning electron microscopy (FE-SEM), and it indicated that after immobilization of OCNF on PCL, the unique nanofiber and nano network was created. Moreover, the physical and chemical properties of samples were examined using X-ray photoelectron spectroscopy (XPS), Fourier transform spectroscopy (FT-IR), thermogravimetric analysis (TGA), water contact angle measurement, and BSA adsorption properties. Furthermore, the cellular responses to PCL scaffold with and without modification by OCNF were examined by seeding rat bone marrow stem cells (RBMSCs) on the fibrous scaffold for assessing cell attachment, cell viability, and proliferation. Thus, the present study focused on preparation and characterization of a membrane and tubular scaffold with a unique nanostructure, which is an excellent candidate for use as a blood vessel scaffold graft.