Both Expression of Cytokines and Posterior Annulus Fibrosus Rupture Are Essential for Pain Behavior Changes Induced by Degenerative Intervertebral Disc: An Experimental Study in Rats
Both Expression of Cytokines and Posterior Annulus Fibrosus Rupture Are Essential for Pain Behavior Changes Induced by Degenerative Intervertebral Disc: An Experimental Study in Rats
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DOI：
10.1002/jor.22494
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发表时间：
2014-02-01
影响因子：
2.8
通讯作者：
Zheng, Zhaomin
中科院分区：
文献类型：
--
作者：
Li, Zemin;Liu, Hui;Zheng, Zhaomin
本研究的目的是分析椎间盘退变与下腰痛(LBP)的关系。采用前、后0.4 mm针环形穿刺法建立大鼠L4/5椎间盘退变模型。在前穿和后穿模型中，磁共振成像(MRI)和组织学分析均显示在穿刺后2周有明显的椎间盘退变。穿孔后3d，髓核内细胞因子表达有不同程度的上调。疼痛行为测试显示，前路穿刺法未引起疼痛行为改变，后路穿刺法在伤后1d~21d出现机械性异位痛觉。此外，背根节细胞因子在背根节(DRG)中的表达在后穿后1周和2周显著增加，但在前穿后无明显变化。这些结果表明，退变椎间盘的NP表达不同程度的炎性细胞因子，后路穿刺术产生机械性痛觉异常。在后路腰椎间盘穿刺术模型中，细胞因子在NP的表达时相与机械性痛觉过敏相一致。退变椎间盘中细胞因子的表达和纤维后环(AF)破裂是疼痛行为改变的重要因素。(C)2013年骨科研究会。由Wiley期刊公司出版，J Orthop Res 32：262-272,2014。
The aim of this study was to analyze the relationship between intervertebral disc degeneration and low back pain (LBP). Rat L4/5 disc degeneration model was established by annular puncture using a 0.4mm needle anteriorly or posteriorly. In both anterior and posterior puncture models, magnetic resonance imaging (MRI) and histological analyses revealed marked disc degeneration 2 weeks after puncture. Cytokine expression was up-regulated in different level in nucleus pulposus (NP) from 3 days after puncture. Pain behavioral tests indicated that the anterior disc puncture did not induce pain behavior changes, whereas the posterior disc puncture resulted in mechanical allodynia from 1 day to 21 days after injury. Besides, cytokine expression was significantly increased in dorsal root ganglion (DRG) at 1 and 2 weeks after posterior puncture, but not after the anterior puncture. These findings indicate the NP of the degenerative disc expresses different levels of inflammatory cytokines, and posterior disc puncture produced mechanical allodynia. The expression phase of cytokines in the NP was accordance with mechanical hyperalgesia in the posterior disc puncture model. Both expression of cytokines and posterior annulus fibrosus (AF) rupture in degenerative intervertebral disc are essential for pain behavior changes. (c) 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:262-272, 2014.