White Marginal Picotee Formation in the Petals of Camellia japonica 'Tamanoura'
White Marginal Picotee Formation in the Petals of Camellia japonica 'Tamanoura'
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DOI：
10.2503/jjshs1.79.207
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发表时间：
2010-04-01
影响因子：
--
通讯作者：
Okubo, Hiroshi
中科院分区：
文献类型：
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作者：
Tateishi, Nobumine;Ozaki, Yukio;Okubo, Hiroshi
通过色素和分子分析，阐明了山茶‘Tamanoura’花瓣中白色边缘皮糖体的表达方式。高效液相色谱分析表明，花瓣红色部分有两种主要的花青素(花青素3-葡萄糖苷和花青素3-半乳糖苷)积累，而花瓣白色部分未检测到花青素，表明花青素生物合成途径可能在花瓣白色部分的某些步骤被阻断。利用RT-PCR技术分析花青素生物合成相关基因的转录水平。大多数基因在‘Tamanoura’花瓣的红色部分和白色部分表达相同，但查尔酮合成酶(CHS)的表达仅在白色微皮部分受到强烈抑制。用5‘和3’RACE方法测定了CjCHS的全长cDNA序列。推导的氨基酸序列与几种木本植物的氨基酸序列有很高的同源性。在‘Tamanoura’花瓣中，红、白两种花瓣部分的DNA扩增产物的长度没有差异，表明在花瓣白色部分的CjCHS中没有发生转座元件插入和DNA重排。
Pigment and molecular analyses were carried out to elucidate how the white marginal picotee in the petals of Camellia japonica 'Tamanoura' is expressed. HPLC analyses showed that two major anthocyanins (cyanidin 3-glucoside and cyanidin 3-galactoside) were accumulated in the red part of the petals of 'Tamanoura', as found in those of wild type C. japonica, whereas no anthocyanins were detected in the white picotee part, indicating that the anthocyanin biosynthetic pathway might be blocked at some steps in the white part. Transcriptional levels of the genes involved in anthocyanin biosynthesis were investigated by RT-PCR. Most genes were equally expressed in both red and white parts of 'Tamanoura' petals, but the expression of chalcone synthase (CHS) was strongly suppressed only in the white picotee part. Full-length cDNA sequence of CjCHS was determined using the 5' and 3' RACE approach. The deduced amino acid sequence of CjCHS shared high homology with those of several woody plants. cDNA RT-PCR and genomic DNA PCR revealed no length differences in PCR products between red and white picotee parts in the petals of 'Tamanoura', suggesting that no insertion of transposable elements and DNA rearrangement occurred in CjCHS in the white part.