How to determine free Gd and free ligand in solution of Gd chelates. A technical note
How to determine free Gd and free ligand in solution of Gd chelates. A technical note
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DOI：
10.1002/cmmi.110
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发表时间：
2006-09-01
影响因子：
--
通讯作者：
Fedeli, Franco
中科院分区：
文献类型：
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作者：
Barge, Alessandro;Cravotto, Giancarlo;Fedeli, Franco
Gd（III）螯合物通常用作磁共振成像应用中的报告探针。它们的使用依赖于这样的假设，即它们的高稳定性确保不释放游离配体和游离Gd 3+离子，这两者对生命系统都是高毒性的。游离Gd 3+离子的存在可能对MR图像中的对比度产生深远影响，因为它们可能形成具有非常高弛豫率的高度水合大分子复合物，从而提供关于给定报告探针诱导的对比度效应程度的错误信息。尽管给定络合物的稳定性可能足够高，但络合步骤可能尚未完成。因此，任何Gd络合物制剂都必须仔细检查游离配体和游离金属离子的含量。本文详细描述了目前使用的允许评估给定Gd络合物溶液中游离金属离子和游离配体的量的程序。版权所有（C）2006约翰威利父子有限公司。
Gd(III) chelates are often used as reporting probes in magnetic resonance imaging applications. Their use relies on the assumption that their high stability ensures against the release of free ligand and free Gd3+ ions, which are both highly toxic to living systems. The presence of free Gd3+ ions may have a profound effect on the contrast in the MR image as they may form highly hydrated, macromolecular complexes endowed with very high relaxivity thus providing erroneous information on the extent of the contrast effect induced by the given reporting probe. Although the stability of a given complex may be high enough, the complexation step could not have been completed. Therefore any Gd complex preparation has to be carefully checked for the content of free ligand and free metal ions. Herein the currently used procedures that allow an assessment of the amounts of free metal ions and free ligand in a solution of a given Gd complex are described in detail. Copyright (C) 2006 John Wiley & Sons Ltd.