Covalent coupling of antibodies to self-assembled monolayers of carboxy-functionalized poly(ethylene glycol): Protein resistance and specific binding of biomolecules
Covalent coupling of antibodies to self-assembled monolayers of carboxy-functionalized poly(ethylene glycol): Protein resistance and specific binding of biomolecules
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DOI：
10.1021/la026327q
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发表时间：
2003-03-04
期刊：
影响因子：
3.9
通讯作者：
Grunze, M
中科院分区：
文献类型：
--
作者：
Herrwerth, S;Rosendahl, T;Grunze, M
我们报告了羧基官能化聚乙二醇烷硫醇[HOOC-CH 2-（OCH 2-CH 2）（n）-O-（CH 2）（11）-SH，n = 22-45]的合成、成膜、蛋白质抗性和特异性抗原结合能力。尽管它的聚合物的字符，分子被发现形成一个密集的自组装单分子层的多晶金，如果从二甲基甲酰胺溶液中吸附。由于其链长分布，预期羧基尾基部分埋在膜内，因此当暴露于纤维蛋白原和免疫球蛋白G（IgG）时，不影响乙二醇部分的蛋白质排斥特性。然而，如果被N-羟基琥珀酰亚胺和N-（3-二甲基氨基丙基）N-乙基碳二亚胺盐酸盐活化，则抗体可以共价偶联至单层。尽管该生物活性层保持了对非特异性纤维蛋白原和IgG吸附的抗性，但其表现出高特异性抗原结合能力。这种高选择性表面的性能进行比较，通过标准的氨基硅烷化学制备的抗体膜。
We report the synthesis, film formation, protein resistance, and specific antigen binding capability of a carboxy-functionalized poly(ethylene glycol) alkanethiol [HOOC-CH2-(OCH2-CH2)(n)-O-(CH2)(11)-SH, n = 22-45]. Despite its polymeric character, the molecule is found to form a densely packed self-assembled monolayer on polycrystalline gold, if adsorbed from dimethylformamide solution. Due to its chain length distribution, the carboxy tailgroups are expected to be partially buried within the film and, thus, do not affect the protein repulsive characteristics of the ethylene glycol moieties when exposed to fibrinogen and immunoglobulin G (IgG). However, if activated by N-hydroxysuccinimide and N-(3-dimethylaminopropyl)N-ethylcarbodiimide hydrochloride, antibodies can be covalently coupled to the monolayer. While resistance to nonspecific fibrinogen and IgG adsorption is maintained for this biologically active layer, it exhibits high specific antigen binding capacity. The performance of this highly selective surface is compared to that of antibody films prepared by standard aminosilane chemistry.