VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation
VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation
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DOI：
10.1007/s00705-012-1273-3
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发表时间：
2012-06-01
影响因子：
2.7
通讯作者：
Johne, Reimar
中科院分区：
文献类型：
--
作者：
Matthijnssens, Jelle;Otto, Peter H.;Johne, Reimar
靶向轮状病毒（RV）蛋白VP 6的间接免疫荧光技术用于区分RV物种。ICTV识别RV种A至E和两个暂定种F和G。已描述了一种潜在的新RV种ADRV-N。用400多个已知的RVD毒株VP 6序列和7个新测定的RVD毒株VP 6序列构建了RVD毒株的系统发育树和同源性频率图。所有RV物种被分成不同的系统发育簇。53%的氨基酸序列截断值坚定地允许RV物种的分化，ADRV-N被暂时分配到一个新的RV物种H（RVH）。
Indirect immunofluorescence techniques targeting the rotavirus (RV) protein VP6 are used to differentiate RV species. The ICTV recognizes RV species A to E and two tentative species, F and G. A potential new RV species, ADRV-N, has been described. Phylogenetic trees and pairwise identity frequency graphs were constructed with more than 400 available VP6 sequences and seven newly determined VP6 sequences of RVD strains. All RV species were separated into distinct phylogenetic clusters. An amino acid sequence cutoff value of 53% firmly permitted differentiation of RV species, and ADRV-N was tentatively assigned to a novel RV species H (RVH).