RADIOSENSITIVITY OF T AND B-LYMPHOCYTES .2. EFFECT OF IRRADIATION ON RESPONSE OF T-CELLS TO ALLOANTIGENS
RADIOSENSITIVITY OF T AND B-LYMPHOCYTES .2. EFFECT OF IRRADIATION ON RESPONSE OF T-CELLS TO ALLOANTIGENS
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DOI：
10.1002/eji.1830040310
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发表时间：
1974-01-01
影响因子：
5.4
通讯作者：
MILLER, JFAP
中科院分区：
文献类型：
--
作者：
SPRENT, J;ANDERSON, RE;MILLER, JFAP
本文通过研究体外照射对亲代胸导管淋巴细胞（a）诱导新生F1小鼠脾肿大和（B）在成年受照F1小鼠增殖能力的抑制作用，研究了T细胞的放射敏感性。通过这些参数，发现小T淋巴细胞具有高度的放射敏感性。经计算，在对所研究的同种异体抗原具有反应性的细胞中，0.3%在暴露于500 r.放射敏感性被认为是间期淋巴细胞死亡的反映。TDL）不同于小T细胞。因此，[3 H]dThd掺入T. TDL测量后1天转移到辐照的F1主机没有废除，虽然降低，暴露剂量高达5000 r; [3 H]dThd掺入测量在2天，但是，大大减少了小得多剂量的照射。鉴于其他地方获得的证据表明，T。TDL对同种异体抗原的作用涉及DNA合成而不涉及细胞分裂，本文的研究是从辐射引起T. TDL在进入DNA合成之前的间期。结果表明，T. TDL对辐射诱导的间期死亡的抵抗力远高于小T细胞。从全身照射前4天的小鼠胸导管淋巴中获得的少量淋巴细胞几乎完全由T细胞组成;这些细胞虽然有活力，但在转移时暴露于同种异体抗原时不能产生增殖反应。
The radiosensitivity of T cells was investigated by studying the effect of irradiationin vitroin suppressing the capacity of parental strain thoracic duct lymphocytes (a) to induce splenomegaly in newborn F1mice, and (b) to proliferate in adult irradiated F1mice as measured by incorporation of tritiated thymidine ([3H]dThd) 4 days after transfer. By these parameters, small T lymphocytes were found to be highly radiosensitive. It was calculated that, of cells with the reactivity to the alloantigens studied, 0.3 % were capable of a proliferative response after exposure to 500 r. Radiosensitivity was considered to be a reflection of lymphocyte death in interphase.The radiosensitivity of H‐2‐activated T cells (T. TDL) differed from that of small T cells. Thus, [3H]dThd incorporation by T. TDL measured 1 day after transfer to irradiated F1hosts was not abolished, although lowered, by exposure to doses as high as 5000 r; [3H]dThd incorporation measured at 2 days, however, was greatly reduced by much smaller doses of irradiation. In view of evidence obtained elsewhere that the response of T. TDL to alloantigens involves DNA synthesis but not cell division, the present studies were interpreted in terms of irradiation causing death of T. TDL in interphase before entry into DNA synthesis. It was concluded that T. TDL were far more resistant to irradiation‐induced interphase death than were small T cells.The small numbers of lymphocytes obtained from thoracic duct lymph of mice exposed to whole body irradiation 4 days before consisted almost entirely of T cells; these cells, although viable, were found incapable of mounting a proliferate response when exposed to alloantigens on transfer.