Two new catechins from Zijuan green tea enhance the fitness and lifespan of Caenorhabditis elegans via insulin-like signaling pathways
Two new catechins from Zijuan green tea enhance the fitness and lifespan of Caenorhabditis elegans via insulin-like signaling pathways
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DOI：
10.1039/d2fo01795d
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发表时间：
2022-08-02
期刊：
影响因子：
6.1
通讯作者：
Bao, Guan-Hu
中科院分区：
文献类型：
--
作者：
Ke, Jia-Ping;Yu, Jing-Ya;Bao, Guan-Hu
绿色茶多酚对人体健康和长寿具有积极的作用。然而，绿色茶的抗衰老特性的知识仅限于主要的儿茶素表没食子儿茶素没食子酸酯（EGCG）。寻找具有较强抗衰老活性的茶叶新成分值得进一步研究。从紫鹃绿色茶中分离鉴定了两种新的儿茶素类化合物，分别命名为紫鹃素E（1）和紫鹃素F（2）。通过高分辨质谱（HR-MS）、核磁共振（NMR）、紫外-可见（UV）、红外（IR）和圆二色性（CD）光谱分析确定了它们的结构，并计算了它们的C-13 NMR和CD数据。我们使用线虫秀丽隐杆线虫（C. elegans）来分析1和2的健康益处和长寿效应。化合物1和2（100 μ M）显著延长了C.分别提高了67.2%和56.0%，延缓了衰老相关的表型衰退，增强了抗逆性，减少了ROS和脂质积累。此外，1和2不影响daf-16、daf-2、sir-2.1和skn-1突变体蠕虫的寿命，这表明它们可能通过胰岛素/IGF和SKN-1/Nrf 2信号通路起作用。同时，化合物1和2在体外也表现出较强的抗氧化活性。表面等离子体共振（SPR）研究表明，紫藤素E和F具有较强的人血清白蛋白（HSA）结合能力。总之，紫鹃素E和F代表了一类新的有价值的茶成分，可以促进健康，并可以开发为潜在的抗衰老饮食疗法。
Green tea polyphenols show positive effects on human health and longevity. However, knowledge of the antiaging properties of green tea is limited to the major catechin epigallocatechin gallate (EGCG). The search for new ingredients in tea with strong antiaging activity deserves further study. Here we isolated and identified two new catechins from Zijuan green tea, named zijuanin E (1) and zijuanin F (2). Their structures were identified by extensive high-resolution mass spectroscopy (HR-MS), nuclear magnetic resonance (NMR), ultraviolet-vis (UV), infrared (IR) and circular dichroism (CD) spectroscopic analyses, and their C-13 NMR and CD data were calculated. We used the nematode Caenorhabditis elegans (C. elegans) to analyze the health benefits and longevity effects of 1 and 2. Compounds 1 and 2 (100 mu M) remarkably prolonged the lifespan of C. elegans by 67.2% and 56.0%, respectively, delaying the age-related decline of phenotypes, enhancing stress resistance, and reducing ROS and lipid accumulation. Furthermore, 1 and 2 did not affect the lifespan of daf-16, daf-2, sir-2.1, and skn-1 mutant worms, suggesting that they might work via the insulin/IGF and SKN-1/Nrf2 signaling pathways. Meanwhile, 1 and 2 also exhibited strong antioxidant activity in vitro. Surface plasmon resonance (SPR) evidence suggests that zijuanins E and F have strong human serum albumin (HSA) binding ability. Together, zijuanins E and F represent a new valuable class of tea components that promote healthspan and could be developed as potential dietary therapies against aging.