Atmospheric Pressure Plasma Surface Modification of Poly(D,L-lactic acid) Increases Fibroblast, Osteoblast and Keratinocyte Adhesion and Proliferation
Atmospheric Pressure Plasma Surface Modification of Poly(D,L-lactic acid) Increases Fibroblast, Osteoblast and Keratinocyte Adhesion and Proliferation
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DOI：
10.1002/ppap.201100139
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发表时间：
2012-05
影响因子：
3.5
通讯作者：
F. Renó;Domenico D'Angelo;G. Gottardi;M. Rizzi;D. Aragno;G. Piacenza;F. Cartasegna;M. Biasizzo;F. Trotta;M. Cannas
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文献类型：
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作者：
F. Renó;Domenico D'Angelo;G. Gottardi;M. Rizzi;D. Aragno;G. Piacenza;F. Cartasegna;M. Biasizzo;F. Trotta;M. Cannas
以1,2-二氨基丙烷和丙烯酸为前驱体，采用常压等离子沉积法制备了P(d，L)LA （PLA）薄膜。傅里叶变换红外光谱（FTIR）和x射线光电子能谱（XPS）表明，这两种不同的等离子体涂层导致PLA表面上高密度的氨基（PLA- nh2）和羧基（PLACOOH）。血浆涂层改变胎牛血清（FBS） PLA表面润湿性和蛋白质吸附，影响3T3小鼠成纤维细胞、MC-3T3 e1小鼠前成骨细胞和HaCaT细胞（人角质形成细胞）的细胞粘附和增殖。特别是这两种涂层都增加了成骨前细胞和角化细胞的粘附，而对成纤维细胞没有影响。此外，48小时后，通过x-8检测，与正常PLA上的细胞相比，在PLA- nh2和PLACOOH上接种的成骨细胞和角质形成细胞的增殖能力明显更高。根据所获得的数据，所描述的大气压等离子沉积可能是骨和皮肤组织工程的一种创新和有用的工具。
An atmospheric pressure plasma deposition for P(d,L)LA (PLA) film was used to modify polymer surface properties using 1,2-diaminopropane and acrylic acid as precursors. These two different plasma coatings result in a high density of amino groups (PLA-NH2) and carboxylic groups (PLACOOH) onto PLA surface as demonstrated by Fourier transform infra-red (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). Plasma coatings modified PLA surface wettability and proteins adsorption from fetal bovine serum (FBS), influencing cell adhesion and proliferation of 3T3 mouse fibroblast, MC-3T3 E1mouse pre-osteoblast, and HaCaT cells (human keratinocytes). In particular both coatings increased pre-osteoblast and keratinocyte adhesion while no effect was observed on fibroblast. Moreover, cell proliferation assessed after 48h by Tox-8 assay was significantly higher for osteoblast cells and keratinocyte seeded onto both PLA-NH2 and PLACOOH compared to cells seeded onto normal PLA. On the basis of the obtained data, the atmospheric pressure plasma deposition described might represent an innovative and useful tool for bone and skin tissue engineering.