Comparative Analyses between Skeletal Muscle miRNAomes from Large White and Min Pigs Revealed MicroRNAs Associated with Postnatal Muscle Hypertrophy.
Comparative Analyses between Skeletal Muscle miRNAomes from Large White and Min Pigs Revealed MicroRNAs Associated with Postnatal Muscle Hypertrophy.
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大白猪和小猪骨骼肌 miRNA 组的比较分析揭示了与产后肌肉肥大相关的 MicroRNA。
DOI：
10.1371/journal.pone.0156780
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Guo Y
中科院分区：
文献类型：
--
作者：
Sheng X;Wang L;Ni H;Wang L;Qi X;Xing S;Guo Y
由microRNAs（miRNAs）调控的骨骼肌肥大的分子机制是复杂的，目前尚不清楚。本研究采用Illumina测序技术对大白色猪（商品猪）和民猪（中国地方猪种）5个生后阶段（生后60、120、150、180和210 d）背最长肌的miRNAomes进行了分析。我们鉴定了734个miRNAs，包括308个注释的miRNAs和426个新的miRNAs，其中307个可以被认为是猪特异性的。通过对两个品种的比较分析，表明生后60和120天是骨骼肌肥大和肌内脂肪积累的重要时期。在两个品种的一个或多个发育阶段，共263个miRNAs的显着差异表达。此外，还测定了各品种中每两个相邻发育阶段之间差异表达的miRNAs。值得注意的是，与其在大白色猪骨骼肌中的表达相比，ssc-miR-204在所有出生后阶段在民猪骨骼肌中的表达显著更高。基于基因本体论和预测靶基因的KEGG通路分析，我们得出结论，ssc-miR-204可能通过调节成肌细胞增殖来影响出生后骨骼肌肥大。本研究结果有助于阐明miRNAs调控骨骼肌肥大的机制，为改善猪肉品质和人类肌病提供有价值的信息。
The molecular mechanism regulated by microRNAs (miRNAs) that underlies postnatal hypertrophy of skeletal muscle is complex and remains unclear. Here, the miRNAomes of longissimus dorsi muscle collected at five postnatal stages (60, 120, 150, 180, and 210 days after birth) from Large White (commercial breed) and Min pigs (indigenous breed of China) were analyzed by Illumina sequencing. We identified 734 miRNAs comprising 308 annotated miRNAs and 426 novel miRNAs, of which 307 could be considered pig-specific. Comparative analysis between two breeds suggested that 60 and 120 days after birth were important stages for skeletal muscle hypertrophy and intramuscular fat accumulation. A total of 263 miRNAs were significantly differentially expressed between two breeds at one or more developmental stages. In addition, the differentially expressed miRNAs between every two adjacent developmental stages in each breed were determined. Notably, ssc-miR-204 was significantly more highly expressed in Min pig skeletal muscle at all postnatal stages compared with its expression in Large White pig skeletal muscle. Based on gene ontology and KEGG pathway analyses of its predicted target genes, we concluded that ssc-miR-204 may exert an impact on postnatal hypertrophy of skeletal muscle by regulating myoblast proliferation. The results of this study will help in elucidating the mechanism underlying postnatal hypertrophy of skeletal muscle modulated by miRNAs, which could provide valuable information for improvement of pork quality and human myopathy.