Interaction of rat ascites hepatoma cells with cultured mesothelial cell layers: a model for tumor invasion.
Interaction of rat ascites hepatoma cells with cultured mesothelial cell layers: a model for tumor invasion.
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发表时间：
1986-05
期刊：
影响因子：
11.2
通讯作者：
H. Akedo;K. Shinkai;M. Mukai;Y. Mori;R. Tateishi;K. Tanaka;R. Yamamoto;T. Morishita
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文献类型：
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作者：
H. Akedo;K. Shinkai;M. Mukai;Y. Mori;R. Tateishi;K. Tanaka;R. Yamamoto;T. Morishita
研究了大鼠腹水肝癌细胞与大鼠肠系膜源性细胞原代培养层的相互作用。从大鼠肠系膜分离肠系膜衍生的细胞，并在含有2倍浓度的氨基酸和维生素并补充有10%小牛血清的Eagle最低必需培养基中培养。原代培养的细胞主要由多边形的间皮细胞组成，形成“铺路石”片。在将肿瘤细胞接种在肠系膜衍生的细胞层上后，观察到三种不同类型的肿瘤细胞生长。1型为间皮细胞层上肿瘤细胞巢堆积形成。2型为间皮细胞层下形成扁平肿瘤细胞岛。在肿瘤细胞接种后2天，在相差显微镜下清楚地观察到这种岛的形成。间皮细胞下肿瘤细胞突起突起偶见。3型为肿瘤细胞悬浮生长。这些类型的肿瘤细胞生长与腹膜内植入肿瘤细胞后观察到的腹膜腔中的肿瘤细胞生长非常相似。当从荷瘤大鼠的血液中回收的肿瘤细胞被接种时，扁平的肿瘤细胞岛形成的频率是从宿主腹腔分离的肿瘤细胞被接种时的15倍。在出现小的扁平肿瘤细胞岛后不久，在细胞层的边缘区域（细胞边缘部分退缩）优先观察到间皮细胞从多边形到梭形的明显形态变化。间皮细胞的回缩不仅通过接种肿瘤细胞来诱导，而且通过加入肿瘤腹水或肿瘤细胞培养物的条件培养基来诱导。通过更换培养基去除效应物，可逆转形态学变化。这些结果表明，在这项研究中描述的系统可以提供一个有用的模型来研究肿瘤细胞的侵袭。
Interactions of rat ascites hepatoma cells with primary cultured layers of rat mesentery-derived cells were studied. The mesentery-derived cells were isolated from rat mesentery and cultured in Eagle's minimum essential medium with a 2-fold concentration of amino acids and vitamins supplemented with 10% calf serum. The primary cultured cells, consisting mainly of mesothelial cells in polygonal shape, forms a "paving stone" sheet. Upon seeding the tumor cells on the mesentery-derived cell layers, three different types of tumor cell growth were observed. Type 1 was the formation of piled-up tumor cell nests on mesothelial cell layers. Type 2 was the formation of flattened tumor cell islands underneath mesothelial cell layers. This island formation was clearly observed under a phase contrast microscope 2 days after the tumor cell seeding. Protrusion of cellular processes of the tumor cells beneath mesothelial cells was occasionally seen. Type 3 was the growth of tumor cells in suspension. These types of tumor cell growth closely resemble those in the peritoneal cavity observed after i.p. implantation of the tumor cells. When the tumor cells recovered from the blood of tumor-bearing rats were seeded, flattened tumor cell islands were formed 15 times more frequently than when the tumor cells isolated from host peritoneal cavity were seeded. Shortly after the appearance of small flattened tumor cell islands, a distinct morphological change of mesothelial cells from polygonal to spindle shape was seen preferentially at the marginal area of the cell layers (a partial retraction of cell edges). The retraction of mesothelial cells was induced not only by seeding the tumor cells but by adding the tumor ascites fluid or the medium conditioned by the tumor cell culture. The morphological change was reversed by changing the culture medium to remove the effectors. These results indicate that the system described in this study can provide a useful model to study tumor cell invasion.