Histidine kinase and response regulator genes as they relate to salinity tolerance in rice
Histidine kinase and response regulator genes as they relate to salinity tolerance in rice
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DOI：
10.1007/s10142-009-0119-x
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发表时间：
2009-08-01
影响因子：
2.9
通讯作者：
Pareek, Ashwani
中科院分区：
文献类型：
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作者：
Karan, Ratna;Singla-Pareek, Sneh L.;Pareek, Ashwani
我们之前的研究表明，Oryza sativa L. Pokkali在无盐条件下保持较高水平的转录本，否则在敏感基因型ir64中，这是在盐度下诱导的。我们想进一步测试这一差异基因调控的假设，在一个给定的应激反应基因家族的成员中，它们具有显著的结构和功能相似性。为此，我们选择研究双组分系统（TCS家族），该系统在激素、非生物应激、光和发育调节下的应激感知和信号转导中起重要作用。我们提供的数据表明，在不同的水稻基因型中，TCS家族的所有成员，包括感觉组氨酸激酶、磷转移蛋白和反应调节因子，都具有不同的转录丰度（在非胁迫和盐度胁迫条件下）。此外，在非胁迫条件下，发现耐盐基因型pokkali中所有TCS成员（RR21除外）的转录本丰度更高。在敏感品种IR64中，盐胁迫诱导的TCS转录本水平相对较高。这些成员中的一些也被发现定位于先前确定的重要的与盐度相关的数量性状位点。基于上述发现，我们认为TCS成员可能在水稻的耐盐性中起着重要作用，可以作为培育耐盐作物植株的有用“候选基因”。
We have previously shown that Oryza sativa L. Pokkali maintains higher levels of transcripts under non-saline conditions, which are otherwise induced under salinity in the sensitive genotype-IR64. We wanted to test this hypothesis of differential gene regulation further, within the members of a given stress responsive gene family, which share significant structural and functional similarities. For this purpose, we chose to work on the two-component system (TCS family) which plays an important role in stress perception and signal transduction under hormonal, abiotic stress, light and developmental regulation. We present data to show that all members of TCS family, including sensory histidine kinases, phosphotransfer proteins and response regulators, are having differential transcript abundance (under both non-stress and salinity stress conditions) in contrasting rice genotypes. Further, under non-stress conditions, transcript abundance for all TCS members (except RR21) was found to be higher in the salt-tolerant genotype-Pokkali. TCS transcripts are otherwise induced by salinity stress to a relatively higher level in the sensitive cultivar IR64. A few of these members were also found to be localised within important salinity-related quantitative trait loci identified earlier. Based on the above findings, we propose that the TCS members may have a significant role in salinity tolerance in rice and can serve as useful 'candidate genes' for raising salinity-tolerant crop plants.