Fabrication of tissue engineered osteochondral grafts for restoring the articular surface of diarthrodial joints.
Fabrication of tissue engineered osteochondral grafts for restoring the articular surface of diarthrodial joints.
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DOI：
10.1016/j.ymeth.2015.03.008
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Tan AR
中科院分区：
文献类型：
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作者：
Roach BL;Hung CT;Cook JL;Ateshian GA;Tan AR
同种异体骨软骨移植是一种有效的软骨修复技术，可修复大面积(10平方厘米)的软骨，但供不应求。具有准确的解剖特征(如轮廓、厚度、结构)的大型双层工程化软骨组织结构可能有利于修复受损的组织。然而，当创建这些骨软骨结构时，保持生物保真度以恢复功能是相关的。在这里，我们描述了一个循序渐进的框架，通过结合高分辨率成像、快速原型制作、印模成型和注射成型，制造出具有正确解剖结构和拓扑结构的大型骨软骨结构。
Osteochondral allograft implantation is an effective cartilage restoration technique for large defects (>10 cm2), though the demand far exceeds the supply of available quality donor tissue. Large bilayered engineered cartilage tissue constructs with accurate anatomical features (i.e. contours, thickness, architecture) could be beneficial in replacing damaged tissue. When creating these osteochondral constructs, however, it is pertinent to maintain biofidelity to restore functionality. Here, we describe a step-by-step framework for the fabrication of a large osteochondral construct with correct anatomical architecture and topology through a combination of high-resolution imaging, rapid prototyping, impression molding, and injection molding.