Impact of particulate microplastics generated from polyethylene terephthalate on gut pathology and immune microenvironments
Impact of particulate microplastics generated from polyethylene terephthalate on gut pathology and immune microenvironments
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DOI：
10.1016/j.isci.2023.106474
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发表时间：
2023-04-06
期刊：
影响因子：
5.8
通讯作者：
Itoh, Yoshito
中科院分区：
文献类型：
--
作者：
Harusato, Akihito;Seo, Wooseok;Itoh, Yoshito
环境微塑料已成为维持地球生态系统的关键问题。在这项研究中，我们从聚对苯二甲酸乙二醇酯（PM-PET）中产生了颗粒状微塑料，并通过使用小鼠模型和实施组织学检查以及结肠免疫细胞和微生物群的多组学分析来研究它们对肠道的影响。因此，组织学方法表明，慢性和生理性低剂量PM-PET暴露分别不影响肠道病理学和粘蛋白屏障。此外，免疫组化分析表明，T细胞，B细胞，巨噬细胞和粒细胞的数量不受PM-PET的影响。然而，RNA-seq分析显示PM-PET对肠道免疫细胞及其代谢中的转录组具有实质性影响，而16 s rRNA宏基因组分析显示微生物群的组成受到适度影响。这些结果表明PM-PET在影响肠道免疫稳态而没有可检测到的炎症方面发挥了意想不到的作用。
Environmental microplastics have emerged as a critical issue in maintaining the planetary ecosystem. In this study, we generated particulate microplastics from polyethylene terephthalate (PM-PET) and investigated their impact in the gut by using mouse models and implementing histological examinations, as well as multi-omics analysis for colonic immune cells and microbiota. As a result, his-tological approaches showed that chronic and physiological low dose exposure of PM-PET did not affect intestinal pathology and mucin barriers, respectively. Moreover, immunohistochemical analysis demonstrated that the numbers of T cells, B cells, macrophages, and granulocytes were not affected by the expo-sure to PM-PET. However, RNA-seq analysis revealed that PM-PET had a sub-stantial impact on the transcriptome in gut immune cells and their metabolisms, while 16s rRNA metagenomic analysis showed that the composition of micro -biota was modestly affected. These results suggest an unexpected role played by the PM-PET in affecting gut immune homeostasis without detectable inflam-mation.