Evaluating the effect of cultivation conditions on palmitoleic acid‐rich lipid production by Scheffersomyces segobiensis DSM 27193
Evaluating the effect of cultivation conditions on palmitoleic acid‐rich lipid production by Scheffersomyces segobiensis DSM 27193
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DOI：
10.1002/bbb.2286
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发表时间：
2021-09
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通讯作者：
Xinhai Zhou;Xinhui Bao;Jie Zhou;F. Xin;Wenming Zhang;Xiujuan Qian;W. Dong;M. Jiang;K. Ochsenreither
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作者：
Xinhai Zhou;Xinhui Bao;Jie Zhou;F. Xin;Wenming Zhang;Xiujuan Qian;W. Dong;M. Jiang;K. Ochsenreither
棕榈油酸（POA）是一种有价值的单不饱和脂肪酸，但很少在微生物脂质中以高比例发现。最近，已证实新分离的产油酵母Schillosomiosegobiensis DSM 27193能够在其细胞内脂质中积累>10%的POA。本研究全面研究了培养基组成和培养条件对该酵母富含POA的脂质积累的影响。结果表明，以酵母膏为氮源，摇瓶发酵可使脂肪含量从3.6%提高到27.3%。将Mg 2+浓度从0.04 g L−1增加到0.16 g L−1导致形成15.3 g L−1的生物质和4.2 g L−1的富含POA的脂质。初始pH、温度和接种量也对富含POA的脂质产生积极影响。在最佳条件下，实现了最高脂质含量31.7%和最高POA含量12.50%，对应于5.3 g L-1脂质和0.7 g L-1 POA。在2.5 L生物反应器中进行的发酵产生28.1 g L−1的生物质，其中脂质含量为39.4%，对应于11.1 g L−1的总脂质和1.4 g L−1的POA。这些结果表明，这种酵母可能是一种潜在的非常适合富含POA的脂质生产的宿主。© 2021化学工业协会和John Wiley & Sons，Ltd
Palmitoleic acid (POA) is a valuable monounsaturated fatty acid but it is rarely found in high proportions in microbial lipids. Recently, the newly isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 has been confirmed to be able to accumulate >10% POA in its intracellular lipids. This study comprehensively investigated the effect of medium composition and cultivation conditions on POA‐rich lipid accumulation by this yeast. The results showed that the lipid content could be improved significantly in shake flask cultivations from 3.6% to 27.3% by using yeast extract as a nitrogen source. Increasing Mg2+ concentration from 0.04 to 0.16 g L−1 resulted in the formation of 15.3 g L−1 of biomass and 4.2 g L−1 of POA‐rich lipids. The initial pH, temperature, and inoculation size also influenced POA‐rich lipid production positively. Under optimal conditions, the highest lipid content of 31.7% with the highest POA content of 12.50% was achieved, corresponding to 5.3 g L−1 of lipids and 0.7 g L−1 of POA. Fermentation conducted in a 2.5 L bioreactor led to 28.1 g L−1 of biomass with 39.4% of lipid content, corresponding to 11.1 g L−1 of total lipids and 1.4 g L−1 of POA. These results suggest that this yeast could be a potentially well‐suited host for POA‐rich lipid production. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd