Collagen-based engineered heart muscle.

Collagen-based engineered heart muscle.
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DOI:
10.1007/978-1-4939-1047-2_15
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发表时间:
2014-01-01
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Zimmermann, Wolfram-Hubertus
Zimmermann, Wolfram-Hubertus
中科院分区:
其他
文献类型:
--
作者:
Tiburcy, Malte;Meyer, Tim;Zimmermann, Wolfram-Hubertus

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近20年来,心肌工程学已经从一个科学怪人发展成为具有广泛应用的主流技术。在许多已发表的方法中,水凝胶似乎构成心肌组织工程和支持器官型发育的优选支架。在这里,我们描述了一个简单的和高度稳健的协议,使用胶原蛋白为基础的水凝胶方法的工程心肌的产生。
Cardiac muscle engineering has evolved over nearly 20 years from a scientific oddity to a mainstream technology with a wide range of applications. Of the many published methods it appears that hydrogels constitute the preferred scaffolds for myocardial tissue engineering and support of organotypic development. Here we describe a simple and highly robust protocol for the generation of engineered heart muscle using a collagen-based hydrogel method.