Prokaryotic diversity of a Tunisian multipond solar saltern
Prokaryotic diversity of a Tunisian multipond solar saltern
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DOI：
10.1007/s00792-008-0154-x
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发表时间：
2008-07-01
期刊：
影响因子：
2.9
通讯作者：
Ammar, Emna
中科院分区：
文献类型：
--
作者：
Baati, Houda;Guermazi, Sonda;Ammar, Emna
在Sfax多池太阳盐沼中，分别从M2（15%）、TS38（25%）和S5(32%) 3个不同盐浓度的池中构建了16S rRNA基因克隆文库。对216个细菌克隆和156个古细菌克隆的16S rRNA基因进行了测序和系统发育分析。细菌和古生菌分别获得44个和67个操作分类单位（otu）。除TS38池塘中发现1个蓝藻OTU外，细菌域内的系统发育类群仅限于拟杆菌门和变形菌门。M2、TS38和S5池的细菌OTUs分别为85.7、26.6%和25.0%。所有古菌16S rRNA基因序列均为Euryarchaeota独有，M2、TS38和S5池塘的OTUs分别为75.0、60.0和66.7%。结果表明，突尼斯多池太阳盐沼蕴藏着以前从未报道过的太阳盐沼新的原核生物多样性。此外，多样性测量表明，随着盐度梯度的升高，细菌多样性减少，古细菌多样性增加，这与先前对海盐系统的观察结果一致。比较分析表明，突尼斯沙鳖的原核多样性高于其他沙鳖。
16S rRNA gene clone libraries were separately constructed from three ponds with different salt concentrations, M2 (15%), TS38 (25%) and S5 (32%), located within a multipond solar saltern of Sfax. The 16S rRNA genes from 216 bacterial clones and 156 archaeal clones were sequenced and phylogenetically analyzed. 44 operational taxonomic units (OTUs) were generated for Bacteria and 67 for Archaea. Phylogenetic groups within the bacterial domain were restricted to Bacteroidetes and Proteobacteria, with the exception that one cyanobacterial OTU was found in the TS38 pond. 85.7, 26.6 and 25.0% of the bacterial OTUs from M2, TS38 and S5 ponds, respectively, are novel. All archaeal 16S rRNA gene sequences were exclusively affiliated with Euryarchaeota. 75.0, 60.0 and 66.7% of the OTUs from, respectively, M2, TS38 and S5 ponds are novel. The result showed that the Tunisian multipond solar saltern harbored novel prokaryotic diversity that has never been reported before for solar salterns. In addition, diversity measurement indicated a decrease of bacterial diversity and an increase of archaeal diversity with rising salinity gradient, which was in agreement with the previous observation for thalassohaline systems. Comparative analysis showed that prokaryotic diversity of Tunisian saltern was higher than that of other salterns previously studied.