Taishan Pinus massoniana Pollen Polysaccharides Enhance Immune Responses in Chickens Infected by Avian Leukosis Virus Subgroup B
Taishan Pinus massoniana Pollen Polysaccharides Enhance Immune Responses in Chickens Infected by Avian Leukosis Virus Subgroup B
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泰山马尾松花粉多糖增强禽白血病病毒B亚群感染鸡的免疫反应
DOI：
10.1080/08820139.2018.1435689
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发表时间：
2018-01-01
影响因子：
2.8
通讯作者：
Zhu, Ruiliang
中科院分区：
文献类型：
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作者：
Yang, Shifa;Li, Guiming;Zhu, Ruiliang
免疫抑制病毒在鸡群中频繁发生，严重破坏了家禽养殖业，可引起免疫抑制和疫苗接种失败。我们前期的研究报道了泰山马尾松花粉多糖（TPPPS）具有免疫调节作用，可以提高疫苗的免疫效果。本研究以禽白血病病毒B亚群（ALV-B）为免疫抑制病毒，在鸡体内人工建立免疫抑制模型，评价TPPPS对鸡免疫应答的免疫调节能力。将免疫抑制鸡随机分为4组（I-IV组），分别以0、100、200和400 mg/kg（每千克鸡）的剂量注射TPPPS。V组以生理盐水作为对照。在7日龄时，从I-V组中随机选择10只鸡接种新城疫减毒疫苗。结果表明，在监测期内，TPPPS显著提高了鸡免疫器官重量、外周血淋巴细胞增殖、CD4+百分比和CD4+/CD8+比值、IL-2和IFN-γ产量以及ALV-B抗体阳性率，且呈剂量依赖性，其中以400 mg/kg TPPPS效果最好。另外，400 mg/kg剂量组的新城疫病毒抗体效价显著高于其他各组。我们观察到TPPPS组的免疫力更强，这表明TPPPS可以作为免疫增强剂来缓解家禽业中ALV-B引起的免疫抑制。
ABSTRACT Immunosuppressive virus, which can cause suppressed immunity and vaccination failure, frequently occurs in chicken flocks and seriously destroys the poultry industry. Our previous studies have reported that Taishan Pinus massoniana pollen polysaccharide (TPPPS) possess immunomodulatory effects and improve the immune effects of vaccines. In this study, avian leukosis virus subgroup B (ALV-B) was chosen as immunosuppressive virus to artificially establish immunosuppressive models in chickens, and the immune modulatory ability of TPPPS on the immune response of chickens was evaluated. Four randomly assigned groups (Group I–IV) of these immunosuppressed chickens were administered with TPPPS at doses of 0, 100, 200, and 400 mg/kg (every kilogram chick), respectively. Group V was administered with saline as control. At seven day old, 10 chickens randomly selected from Group I–V were inoculated with the attenuated Newcastle disease (ND) vaccine. The results showed that during the monitoring period, TPPPS significantly enhanced weight of immune organs, peripheral lymphocyte proliferation, the percentage of CD4+ and the ratio of CD4+/CD8+, IL-2 and IFN-γ production, and ALV-B antibody positive rate of chickens in a dose-dependent manner, with 400 mg/kg TPPPS being the most effective. In addition, the antibody titer against Newcastle disease virus (NDV) in Group IV with 400 mg/kg was significantly higher than those in other groups. We observed the stronger immunity in the TPPPS group, which indicates that TPPPS could be used as an immunoenhancer to relieve immunosuppression caused by ALV-B in the poultry industry.