Modified P elements that mimic the P cytotype in Drosophila melanogaster.
Modified P elements that mimic the P cytotype in Drosophila melanogaster.
复制标题
模拟果蝇 P 细胞型的修饰 P 元件。
DOI：
10.1093/genetics/123.4.815
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发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
Engels,WR
中科院分区：
文献类型：
--
作者：
Robertson,HM;Engels,WR
果蝇转座因子P家族的活性主要受一种称为P细胞型的细胞状况调节。据推测，P细胞型依赖于P元件编码的转位和切除抑制因子。我们提供了支持这一观点的证据，表明两个修饰的P元件，每个都有影响第四个转座酶外显子的病变，模拟了大多数P细胞型效应。这些元素是通过两种能够检测单个P元素抑制的敏感分析来鉴定的。一种试验利用了某些P元素插入突变在烧焦猪鬃位点的细胞型依赖基因表达。另一种方法是通过异常稳定的基因组P元件δ 2-3（99B）抑制转座酶活性，该元件通常在生发组织和体细胞组织中产生转座酶。P细胞样效应包括抑制雪胚系超突变、雪体细胞嵌合、蛹致死性和性腺发育不良不育。然而，与P细胞型不同，在抑制遗传中不存在互反交叉效应。
Activity of the P family of transposable elements in Drosophila melanogaster is regulated primarily by a cellular condition known as P cytotype. It has been hypothesized that P cytotype depends on a P element-encoded repressor of transposition and excision. We provide evidence in support of this idea by showing that two modified P elements, each with lesions affecting the fourth transposase exon, mimic most of the P cytotype effects. These elements were identified by means of two sensitive assays capable of detecting repression by a single P element. One assay makes use of cytotype-dependent gene expression of certain P element insertion mutations at the singed bristle locus. The other measures suppression of transposase activity from the unusually stable genomic P element, delta 2-3(99B), that normally produces transposase in both germinal and somatic tissues. The P cytotype-like effects include suppression of snw germline hypermutability, snw somatic mosaicism, pupal lethality, and gonadal dysgenic sterility. Unlike P cytotype, however, there was no reciprocal cross effect in the inheritance of repression.