Cotton BCP genes encoding putative blue copper-binding proteins are functionally expressed in fiber development and involved in response to high-salinity and heavy metal stresses.
Cotton BCP genes encoding putative blue copper-binding proteins are functionally expressed in fiber development and involved in response to high-salinity and heavy metal stresses.
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DOI：
10.1111/j.1399-3054.2010.01420.x
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发表时间：
2011
影响因子：
6.4
通讯作者：
Xiang-Mei Ruan;Fang Luo;Deng-Di Li;Jie Zhang;Zhi–Hao Liu;Wenliang Xu;Gengqing Huang;Xue-Bao Li
中科院分区：
文献类型：
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作者：
Xiang-Mei Ruan;Fang Luo;Deng-Di Li;Jie Zhang;Zhi–Hao Liu;Wenliang Xu;Gengqing Huang;Xue-Bao Li
铜对低浓度的植物是至关重要的，但对高浓度的植物来说却是剧毒的。植物已经进化出一系列机制来防止铜的过剩或短缺带来的后果。这些机制需要与铜相互作用的蛋白质参与铜的运输。蓝铜结合蛋白(BCP)是一类含有一个蓝铜结合结构域的铜蛋白，它与单一的I型铜结合。为了研究BCPs在植物发育和响应逆境中的作用，我们从棉花(Gossypium Hirsutom)中分离了9个编码蓝色铜结合蛋白(GhBCPs)的cDNAs。同时，从棉花基因组中分离到4个相应的基因(包括GhBCP1-GhBCP4)，在其保守位置插入了一个内含子。定量逆转录聚合酶链式反应(RT-PCR)分析表明，这9个GhBCP基因在棉花组织中存在差异表达。其中，GhBCP1和GhBCP4主要在纤维中表达，而GhBCP2和GhBCP3在纤维中的表达水平较高。这四个基因在纤维伸长早期有较强的表达，但随着纤维的进一步发育而急剧下降。此外，铜(2+)、锌(2+)、高盐胁迫和干旱胁迫上调了这些GhBCP基因在纤维中的表达，而铝(3+)处理下调了这些基因在纤维中的表达。在酵母菌中过表达GhBCP1和GhBCP4显著提高了细胞在铜、锌和高盐胁迫下的生长速度。这些结果表明，这些GhBCP可能参与了棉花纤维发育的调控和对高盐度和重金属胁迫的响应。
Copper is vitally required for plants at low concentrations but extremely toxic for plants at elevated concentrations. Plants have evolved a series of mechanisms to prevent the consequences of the excess or deficit of copper. These mechanisms require copper-interacting proteins involved in copper trafficking. Blue copper-binding proteins (BCPs) are a class of copper proteins containing one blue copper-binding domain binding a single type I copper. To investigate the role of BCPs in plant development and in response to stresses, we isolated nine cDNAs encoding the putative blue copper-binding proteins (GhBCPs) from cotton (Gossypium hirsutum). Meanwhile, four corresponding genes (including GhBCP1-GhBCP4), which contain a single intron inserted in their conserved position, were isolated from cotton genome. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that the nine GhBCP genes are differentially expressed in cotton tissues. Among them, GhBCP1 and GhBCP4 were predominantly expressed in fibers, while the transcripts of GhBCP2 and GhBCP3 were accumulated at relatively high levels in fibers. These four genes were strongly expressed in early fiber elongation, but dramatically declined with further fiber development. In addition, these GhBCP genes were upregulated in fibers by Cu(2+) , Zn(2+) , high-salinity and drought stresses, but downregulated in fibers by Al(3+) treatment. Overexpression of GhBCP1 and GhBCP4 in yeast (Schizosaccharomyces pombe) significantly increased the cell growth rate under Cu(2+) , Zn(2+) and high-salinity stresses. These results suggested that these GhBCPs may participate in the regulation of fiber development and in response to high-salinity and heavy metal stresses in cotton.