Biocontrol of Pythium root rot on lisianthus using a new dark septate endophytic fungus Hyaloscypha variabilis J1PC1
Biocontrol of Pythium root rot on lisianthus using a new dark septate endophytic fungus Hyaloscypha variabilis J1PC1
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DOI：
10.1007/s10658-022-02459-0
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发表时间：
2022-02
影响因子：
1.8
通讯作者：
M. Marian;Yusuke Takashima;W. Harsonowati;H. Murota;K. Narisawa
中科院分区：
文献类型：
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作者：
M. Marian;Yusuke Takashima;W. Harsonowati;H. Murota;K. Narisawa
根腐病是日本百合最具破坏性的病害之一。目前的根腐病防治策略在防治上还存在不足，特别是在水培栽培的桔梗上。本研究旨在探讨暗隔内生真菌（dark - sepate endophytic， DSE）抑制不规则叶青菌引起的根腐病的潜力。在筛选的10个DSE中，有7个DSE对植株的生长产生了积极的影响，因此在无菌双板试验中被选中进行第一次筛选。因此，分离物J1PC1表现出一致的疾病抑制作用，因此被选为小型和大型水培试验以及商品土壤盆栽试验的最终候选物。与未处理对照相比，J1PC1处理显著降低了茎部疾病严重程度和发病率。J1PC1防治效果达50-70%，可生产出畅销的百叶菊。J1PC1对p无直接拮抗和寄生作用。不规则，表明可能涉及其他机制。利用种特异性引物进行Real-time PCR，即使在植株处理3个月后，也能成功地从根组织中检测到J1PC1。通过培养、形态学和基于内部转录间隔的分子分析，J1PC1被鉴定为可变透明丝菌（hyaloscypha variabilis）。综上所述，我们的研究结果强烈表明J1PC1是一种很有前途的防霉根腐病剂。
Pythium root rot is one of the most destructive diseases on lisianthus in Japan. Current management strategies against Pythium root rot are insufficient in controlling the disease especially on hydroponically grown lisianthus. This study aimed to explore the potential of dark septate endophytic (DSE) fungi to suppress the root rot disease caused byPythium irregulare. Among the 10 DSE screened for their pathogenicity on lisianthus, seven DSE produced a positive effect on shoot growth and were thus selected for the first screening in an axenic dual-plate assay. Accordingly, the isolate J1PC1 displayed consistent disease suppression and was thus selected as a final candidate for evaluation in small- and large-scale hydroponic experiments as well as a pot experiment using commercial soil. Shoot disease severity and disease incidence were significantly reduced by J1PC1 treatment compared with that in the untreated control. The control efficacy of J1PC1 reached 50–70%, consequently producing highly marketable lisianthus plants. J1PC1 showed no signs of direct antagonism nor parasitism onP. irregulare,suggesting that other mechanisms might be involved. Real-time PCR using species-specific primers successfully detected J1PC1 from the root tissues even after 3 months of plant treatment. Using cultural, morphological, and internal transcribed spacer-based molecular analysis, J1PC1 was identified asHyaloscypha variabilis. Collectively, our results strongly indicate that J1PC1 is a promising biocontrol agent for Pythium root rot.