Synergistic effect between LH and estrogen in the acceleration of cumulus expansion via GPR30 and EGFR pathways.
Synergistic effect between LH and estrogen in the acceleration of cumulus expansion via GPR30 and EGFR pathways.
复制标题
LH 和雌激素通过 GPR30 和 EGFR 途径加速卵丘扩张的协同作用
DOI：
10.18632/aging.104029
复制
发表时间：
2020-10-28
期刊：
影响因子：
--
通讯作者：
Ma B
中科院分区：
文献类型：
--
作者：
Liu J;Yao R;Lu S;Xu R;Zhang H;Wei J;Zhao C;Tang Y;Li C;Liu H;Zhao X;Wei Q;Ma B
雌激素膜受体GPR 30（也称为G蛋白偶联受体30）最近已被证明参与卵母细胞成熟和卵丘扩张的调节。然而，GPR 30的表达是否受促性腺激素刺激的调节，以及它如何参与成熟过程的调节仍不清楚。本研究探讨了促黄体生成素（LH）和17β-雌二醇（17β-E2）协同促进小鼠卵丘卵母细胞复合体（COCs）成熟过程中表皮生长因子（EGF）反应的机制。采用实时定量PCR、免疫印迹和免疫荧光染色检测GPR 30、EGFR和EGF样生长因子的表达和分布。用Lyso-Tracker Red标记法检测卵泡颗粒细胞（FGC）中溶酶体的活性。COCs体外成熟16 h后观察卵丘扩张情况。我们发现EGF样生长因子通过抑制溶酶体中的蛋白质降解来传递LH信号以增加GRP 30水平。同时，17β-E2通过刺激GPR 30信号通路，增加EGF受体水平，增强COCs对EGF信号的反应能力，从而促进卵丘扩张。本研究揭示了LH和雌激素在卵母细胞成熟过程中对卵丘扩张的协同调控机制。
The estrogen membrane receptor GPR30 (also known as G-protein coupled receptor 30) has recently been shown to be involved in the regulation of oocyte maturation and cumulus expansion. However, whether GPR30 expression is regulated by gonadotropin stimulation and how it participates in the regulation of the maturation process is still not clear. In this study, we explored the mechanism underlying the synergy between luteinizing hormone and 17β-estradiol (17β-E2) to improve the epidermal growth factor (EGF) response in cumulus oocyte complexes (COCs) during oocyte maturation in mice. The expression and distribution of GPR30, EGFR, and EGF-like growth factors were examined by real-time quantitative PCR, western blot, and immunofluorescence staining. Lyso-Tracker Red labeling was performed to detect the lysosomal activity in follicle granular cells (FGCs). Cumulus expansion of COCs was evaluated after in vitro maturation for 16 h. We found that EGF-like growth factors transmit LH signals to increase GRP30 levels by inhibiting protein degradation in lysosomes. Meanwhile, 17β-E2 stimulates the GPR30 signaling pathway to increase EGF receptor levels, enhancing the response ability of EGF signaling in COCs and thus promoting cumulus expansion. In conclusion, our study reveals the synergistic mechanism between LH and estrogen in the regulation of cumulus expansion during oocyte maturation process.
登录
查看更多内容
DOI：
10.1186/s12958-015-0112-2
发表时间：
2015-10-06
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Blaha M;Nemcova L;Kepkova KV;Vodicka P;Prochazka R
通讯作者：
Prochazka R
G蛋白偶联的雌激素受体GPER在健康和疾病中。
DOI：
10.1038/nrendo.2011.122
发表时间：
2011-08-16
期刊：
Nature reviews. Endocrinology
影响因子：
--
作者：
通讯作者：
--
NHERF1 是一种新型 GPER 相关蛋白，可增加 ER 阳性乳腺癌中 GPER 的稳定性和活化
影响因子：
--
作者：
Meng R;Qin Q;Xiong Y;Wang Y;Zheng J;Zhao Y;Tao T;Wang Q;Liu H;Wang S;Jiang WG;He J
通讯作者：
He J
影响因子：
5.3
作者：
Bustamante HA;González AE;Cerda-Troncoso C;Shaughnessy R;Otth C;Soza A;Burgos PV
通讯作者：
Burgos PV
影响因子：
5.3
作者：
Hsieh, Minnie;Lee, Daekee;Conti, Marco
通讯作者：
Conti, Marco