Microfluidic biochip for blood cell lysis
Microfluidic biochip for blood cell lysis
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DOI：
10.1016/s1872-2040(07)60017-6
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发表时间：
2006-11-01
影响因子：
1.2
通讯作者：
Cai Haoyuan
中科院分区：
文献类型：
--
作者：
Chen Xing;Cui Dafu;Cai Haoyuan
基于微机电系统（MEMS）技术，设计并制作了一种用于血细胞裂解的三明治流微流控生物芯片。将细胞溶液夹在化学试剂溶液之间，并将两者引入生物芯片。然后，两种溶液流过生物芯片的微通道。最后，在连续流动过程中，通过细胞溶液和化学试剂溶液的充分混合来实现细胞裂解。以抗凝大鼠血为细胞样品，分别以胍和Triton X-100为裂解剂，比较两种试剂对细胞的裂解效果。以胍为裂解剂，分析了细胞浓度和流速对细胞裂解的影响。当裂解液的流速比细胞样品的流速快很多时，血细胞可以在几分钟内裂解。当裂解液和细胞样品的流量按上述比例同时增加时，血细胞在微流控芯片中可以得到快速裂解。将细胞裂解微流控芯片与细胞分离芯片和脱氧核糖核酸提取芯片集成，可实现复杂生物样品的完全前处理，为实现小型化全分析系统奠定基础。
Based on micro-electro-mechanical system (MEMS) technology, a sandwich flow microfluidic biochip for blood cell lysis was designed and fabricated. Cell solution was sandwiched in between chemical reagent solution, and both were introduced into biochip. Then the two solutions were flowing through the microchannel of the biochip. Finally, cells lysis was brought about by full blend of cell solution and chemical reagent solution during continuous flow. Rat blood with anticoagulant was cell sample, while guanidine and Triton X-100 were used as the lysing reagent, respectively, and the effects of the two reagents on cell lysis were compared. The effects of cell concentration and flow rate on cell lysis were analyzed by using guanidine as the lysing reagent. Blood cells can be lysed in a few minutes when the flow rate of lysing reagent was faster than the flow rate of cell sample so much. Blood cells can be rapid lysed in microfluidic biochip when the flow rates of lysing reagent and cell sample were increased simultaneously with above proportion. The sandwich-type microfluidic biochip for cell lysis potentially integrated with the biochip for cell separation and the biochip for deoxyribonucleic acid extraction could enable complete pretreatment of complex biologic samples, which can lay the foundation for realization of miniaturized total analysis system.