Study on the biodegradation of crude oil by free and immobilized bacterial consortium in marine environment.
Study on the biodegradation of crude oil by free and immobilized bacterial consortium in marine environment.
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海洋环境中游离和固定化菌群生物降解原油的研究
DOI：
10.1371/journal.pone.0174445
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Bao M
中科院分区：
文献类型：
--
作者：
Chen Q;Li J;Liu M;Sun H;Bao M
从浙江沿海分离到5株细菌，分别为铜绿假单胞菌ASW-1、铜绿假单胞菌ASW-2、产碱杆菌ASW-3、产碱杆菌ASS-1和芽孢杆菌ASS-2。5株菌的混合植物群在7天内对1%（w/v）原油的降解率为75.1%。采用海藻酸钙-活性炭包埋载体对细菌复合体进行包埋。固定化细胞在培养温度、初始pH值、培养基盐度和原油浓度等环境因子的变化中表现出比游离细胞更好的活性。与游离菌相比，固定化菌对原油的降解速度明显加快。微生物菌群对正构烷烃的降解性能优于多环芳烃。固定化对正构烷烃的降解率（31.9%）明显高于对多环芳烃的降解率（1.9%）。
Five strains of bacteria, namely, Exiguobacterium sp. ASW-1, Pseudomonas aeruginosa strain ASW-2, Alcaligenes sp. ASW-3, Alcaligenes sp. ASS-1, and Bacillus sp. ASS-2, were isolated from the Zhejiang coast in China. The mixed flora of the five strains performed well with degrading 75.1% crude oil (1%, w/v) in 7 days. The calcium alginate—activated carbon embedding carrier was used to immobilize bacterial consortium. Immobilized cells performed better than free ones in variations of environmental factors containing incubated temperature, initial pH, salinity of the medium and crude oil concentration. The degradation process of crude oil by immobilized bacteria was accelerated compared with that of the free ones. Bacterial consortium showed better performance on biodegradation of normal alkanes than that of PAHs. Improvement of immobilization on the biodegradation efficiency of normal alkanes (31.9%) was apparently high than that of PAHs (1.9%).