Proteomic interrogation of the meninges reveals the molecular identities of structural components and regional distinctions along the CNS axis.
Proteomic interrogation of the meninges reveals the molecular identities of structural components and regional distinctions along the CNS axis.
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DOI：
10.1186/s12987-023-00473-w
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发表时间：
2023-10-19
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
--
作者：
脑膜包围着大脑和脊髓，提供物理保护，同时也是神经免疫活动的利基。尽管具有基质性质，但其复杂的细胞和细胞外组成尚不清楚，脑膜是否沿神经轴变化尚不清楚。因此，开展了研究以阐明正常成年Biozzi ABH小鼠脑和脊髓脑膜的蛋白质组成和结构组织。首先，我们进行了自下而上的蛋白质组学研究。脑和脊髓水平的主要蛋白质包括II型胶原和II型角蛋白，分别代表细胞外基质(ECM)和细胞骨架类别。虽然绝大多数检测到的总蛋白是在两个脑膜部位共享的，但在大脑中唯一检测到的蛋白质比在脊椎中检测到的更多。当总蛋白按细胞间隔细分时，也可以看到这种模式，但在ECM类别的情况下，脑和脊膜中的独特蛋白数量几乎相同，并且V型和III型胶原仅在脊柱中登记。定量分析显示，几种胶原蛋白和细胞骨架蛋白在脑和脊髓膜中的表达存在差异。对仍包裹在骨骼中的全脑和脊髓切片进行高分辨率免疫荧光和免疫金扫描电子显微镜观察，发现蛋白质组学检测到的主要蛋白质，并强调它们与不同形状的蛛网膜小梁的细胞和细胞外成分有关。Western blotting与蛋白质组学和免疫组织学分析相一致，加强了脑和脊膜中蛋白质的差异外观。结果可能反映了管理保护和/或神经免疫功能的脑膜的地区差异。网上版载有补充材料，可在10.1186/s12987-023-00473-w查阅。
The meninges surround the brain and spinal cord, affording physical protection while also serving as a niche of neuroimmune activity. Though possessing stromal qualities, its complex cellular and extracellular makeup has yet to be elaborated, and it remains unclear whether the meninges vary along the neuroaxis. Hence, studies were carried-out to elucidate the protein composition and structural organization of brain and spinal cord meninges in normal, adult Biozzi ABH mice. First, shotgun, bottom-up proteomics was carried-out. Prominent proteins at both brain and spinal levels included Type II collagen and Type II keratins, representing extracellular matrix (ECM) and cytoskeletal categories, respectively. While the vast majority of total proteins detected was shared between both meningeal locales, more were uniquely detected in brain than in spine. This pattern was also seen when total proteins were subdivided by cellular compartment, except in the case of the ECM category where brain and spinal meninges each had near equal number of unique proteins, and Type V and type III collagen registered exclusively in the spine. Quantitative analysis revealed differential expression of several collagens and cytoskeletal proteins between brain and spinal meninges. High-resolution immunofluorescence and immunogold-scanning electronmicroscopy on sections from whole brain and spinal cord – still encased within bone –identified major proteins detected by proteomics, and highlighted their association with cellular and extracellular elements of variously shaped arachnoid trabeculae. Western blotting aligned with the proteomic and immunohistological analyses, reinforcing differential appearance of proteins in brain vs spinal meninges. Results could reflect regional distinctions in meninges that govern protective and/or neuroimmune functions. The online version contains supplementary material available at 10.1186/s12987-023-00473-w.
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影响因子：
16.6
作者：
Chen Y;Yang S;Lovisa S;Ambrose CG;McAndrews KM;Sugimoto H;Kalluri R
通讯作者：
Kalluri R
影响因子：
4.8
作者：
Coles, Jonathan A.;Stewart-Hutchinson, Phillip J.;Brewer, James M.
通讯作者：
Brewer, James M.
DOI：
10.1126/science.abf9277
发表时间：
2021-07-23
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Brioschi S;Wang WL;Peng V;Wang M;Shchukina I;Greenberg ZJ;Bando JK;Jaeger N;Czepielewski RS;Swain A;Mogilenko DA;Beatty WL;Bayguinov P;Fitzpatrick JAJ;Schuettpelz LG;Fronick CC;Smirnov I;Kipnis J;Shapiro VS;Wu GF;Gilfillan S;Cella M;Artyomov MN;Kleinstein SH;Colonna M
通讯作者：
Colonna M
影响因子：
5.6
作者：
Arseni L;Lombardi A;Orioli D
通讯作者：
Orioli D
影响因子：
2.8
作者：
Bonifasi-Lista, C;Lake, SP;Weiss, JA
通讯作者：
Weiss, JA