Recombinant Antibodies in Basic Neuroscience Research.
Recombinant Antibodies in Basic Neuroscience Research.
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DOI：
10.1002/cpns.106
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发表时间：
2020-12
影响因子：
--
通讯作者：
Trimmer JS
中科院分区：
文献类型：
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作者：
Trimmer JS
基础神经科学研究使用抗体作为关键试剂来标记、捕获和调节感兴趣的蛋白质的功能。抗体是免疫球蛋白。重组抗体是免疫球蛋白，其核酸编码区或其片段已被克隆到允许无限生产的表达质粒中。重组抗体提供了许多优于常规抗体的优点，包括它们通过DNA测序的明确鉴定和数字存档、可靠的表达、作为DNA序列和作为质粒的容易和可靠的分布，以及许多形式的工程改造以增强它们的效用的机会。重组抗体以许多不同的形式存在，每种形式都为神经科学研究应用提供了潜在的优点和缺点。我提供了重组抗体及其发展的概述。它们作为有价值的试剂在基础神经科学研究中的新兴用途的例子也进行了讨论。这些实例中的许多在不能用常规抗体进行的创新实验方法中采用重组抗体。
Basic neuroscience research employs antibodies as key reagents to label, capture, and modulate the function of proteins of interest. Antibodies are immunoglobulin proteins. Recombinant antibodies are immunoglobulin proteins whose nucleic acid coding regions, or fragments thereof, have been cloned into expression plasmids that allow for unlimited production. Recombinant antibodies offer many advantages over conventional antibodies including their unambiguous identification and digital archiving via DNA sequencing, reliable expression, ease and reliable distribution as DNA sequences and as plasmids, and the opportunity for numerous forms of engineering to enhance their utility. Recombinant antibodies exist in many different forms, each of which offers potential advantages and disadvantages for neuroscience research applications. I provide an overview of recombinant antibodies and their development. Examples of their emerging use as valuable reagents in basic neuroscience research are also discussed. Many of these examples employ recombinant antibodies in innovative experimental approaches that cannot be pursued with conventional antibodies.