MicroRNA-155 plays critical effects on Th2 factors expression and allergic inflammatory response in type-2 innate lymphoid cells in allergic rhinitis
MicroRNA-155 plays critical effects on Th2 factors expression and allergic inflammatory response in type-2 innate lymphoid cells in allergic rhinitis
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MicroRNA-155对过敏性鼻炎中2型先天淋巴细胞的Th2因子表达和过敏性炎症反应发挥关键作用
DOI：
10.26355/eurrev_201905_17911
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发表时间：
2019-05-01
影响因子：
3.3
通讯作者：
Wang, M-Q
中科院分区：
文献类型：
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作者：
Zhu, Y-Q;Liao, B.;Wang, M-Q
目的：变应性鼻炎（AR）是一种慢性炎症性疾病。本研究旨在研究microRNA-155（miR-155）在AR的2型先天淋巴细胞（ILC 2）中的作用。采用定量真实的时间-聚合酶链反应（qRTPCR）测定鼻粘膜组织中miR-155、白细胞介素-25（IL-25）和白细胞介素-33（IL-33）的mRNA表达。通过注射小鼠IL-33建立AR模型。使用流式细胞术定量ILC 2的频率。将MiR-155阿戈米尔或阿托米尔鼻内施用至小鼠。分别用定量实时PCR（qRT-PCR）、酶联免疫吸附试验（ELISA）或Western印迹法测定miR-155和辅助性T细胞2（Th 2）细胞因子。结果：与对照组相比，miR-155 mRNA表达水平显著降低（P
OBJECTIVE: Allergic rhinitis (AR) is a chronic inflammatory disease. This study aimed to investigate the role of microRNA-155 (miR-155) in type-2 innate lymphoid cells (ILC2s) on AR.PATIENTS AND METHODS: Nasal mucosa tissues and peripheral blood samples were collected. mRNA expression of miR-155, interleukin-25 (IL-25), and interleukin-33 (IL-33) in nasal mucosa tissues was determined using quantitative Real Time-Polymerase Chain Reaction (qRTPCR). The AR model was established by injecting with murine IL-33. The frequency of ILC2s was quantified using flow cytometry. MiR-155 agomir or antagomir was intranasally administrated to mice. MiR-155 and helper T cell 2 (Th2) cytokines were measured with quantitative Real-time PCR (qRT-PCR), enzyme-linked immunosorbent assay (ELISA) or Western blotting, respectively. Hematoxylin and eosin (HE) staining was used for the histopathological examination.RESULTS: Compared with controls, mRNA levels miR-155 (p