FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques.
FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques.
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DOI：
10.1007/s11481-017-9775-0
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Jain P
中科院分区：
文献类型：
--
作者：
Dave RS;Sharma RK;Muir RR;Haddad E;Gumber S;Villinger F;Nehra AP;Khan ZK;Wigdahl B;Ansari AA;Byrareddy SN;Jain P
脑脊液（CSF）通过淋巴引流途径引流。该淋巴通路将中枢神经系统（CNS）连接到颈部淋巴结（CLN）。当CSF经由硬脑膜和鼻粘膜引流至CLN时，T细胞和抗原呈递细胞从蛛网膜下腔沿沿着通过筛板。人类免疫缺陷病毒（HIV）也可沿沿着该途径从CNS流出。因此，从CNS流出的HIV可能在CLN内蓄积。为此，我们分析了慢性感染猴免疫缺陷病毒（SIV）的恒河猴分离的CLN。我们检测到显着积累的SIV内CLN。在CLN滤泡区域内的滤泡树突状细胞（FDC）上观察到SIV病毒粒子捕获。此外，当FDC与生发中心内的B细胞相互作用时，SIV抗原形成免疫复合物。随后这些B细胞与CD4+ T滤泡辅助细胞（TFH）的相互作用导致后者感染。值得注意的是，CLN内73%至90%的TFH细胞对SIV p27抗原呈阳性。因此，看起来FDC不仅保留SIV，它们还将它们（经由B细胞）传输到这些CLN内的TFH。这种相互作用导致CLN中TFH的感染。基于这些观察结果，我们推断，在CLN的FDCs有一个新的作用，SIV诱捕与病毒贩运的影响。
Cerebrospinal fluid (CSF) drains via the lymphatic drainage pathway. This lymphatic pathway connects the central nervous system (CNS) to the cervical lymph node (CLN). As the CSF drains to CLN via the dural and nasal lymphatics, T cells and antigen presenting cells pass along the channels from the subarachnoid space through the cribriform plate. Human immunodeficiency virus (HIV) may also egress from the CNS along this pathway. As a result, HIV egressing from the CNS may accumulate within the CLN. Towards this objective, we analyzed CLNs isolated from rhesus macaques that were chronically-infected with simian immunodeficiency virus (SIV). We detected significant accumulation of SIV within the CLNs. SIV virion trapping was observed on follicular dendritic cells (FDCs) localized within the follicular regions of CLNs. In addition, SIV antigens formed immune complexes when FDCs interacted with B cells within the germinal centers. Subsequent interaction of these B cells with CD4+ T follicular helper cells (TFHs) resulted in infection of the latter. Of note, 73% to 90% of the TFHs cells within CLNs were positive for SIV p27 antigen. As such, it appears that not only do the FDCs retain SIV they also transmit them (via B cells) to TFHs within these CLNs. This interaction results in infection of TFHs in the CLNs. Based on these observations, we infer that FDCs within the CLNs have a novel role in SIV entrapment with implications for viral trafficking.
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DOI：
10.4049/jimmunol.1103138
发表时间：
2012-04-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hong JJ;Amancha PK;Rogers K;Ansari AA;Villinger F
通讯作者：
Villinger F
DOI：
10.1084/jem.20101773
发表时间：
2011-05-09
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Fahey LM;Wilson EB;Elsaesser H;Fistonich CD;McGavern DB;Brooks DG
通讯作者：
Brooks DG
影响因子：
32.4
作者：
Heesters, Balthasar A.;Chatterjee, Priyadarshini;Carroll, Michael C.
通讯作者：
Carroll, Michael C.
影响因子：
17.1
作者：
Iliff JJ;Wang M;Liao Y;Plogg BA;Peng W;Gundersen GA;Benveniste H;Vates GE;Deane R;Goldman SA;Nagelhus EA;Nedergaard M
通讯作者：
Nedergaard M
影响因子：
3.2
作者：
Hollenbach, Rebecca;Sagar, Divya;Khan, Zafar K.;Callen, Shannon;Yao, Honghong;Shirazi, Jasmine;Buch, Shilpa;Jain, Pooja
通讯作者：
Jain, Pooja