Extracorporeal liver support:: Porcine or human cell based systems?
Extracorporeal liver support:: Porcine or human cell based systems?
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DOI：
10.1177/039139880202501017
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发表时间：
2002-10-01
影响因子：
1.7
通讯作者：
Neuhaus, R
中科院分区：
文献类型：
--
作者：
Gerlach, JC;Zeilinger, K;Neuhaus, R
原代猪肝细胞用于体外肝支持的初步临床结果正在审查中，因为细胞来源存在争议。根据欧洲移植的数据，由于脂肪变性或肝硬化等因素，20-25%的移植供体肝脏未被移植。这个数字与需要桥接治疗的急性肝衰竭患者的数量相对应。从移植废物中提取的原代人肝细胞可以在生物反应器中分离、纯化和维持，为基于细胞的体外肝支持治疗提供了另一种选择。四室生物反应器可使保存和隔离损伤在毛细血管膜交织的三维网络中恢复，并具有完整的氧合，使这些废弃供体器官的肝细胞可用于临床应用。可以避免患者接触其他动物源性生物基质和胎牛血清。体外培养阶段的开始允许细胞稳定，质量控制，并且无需冷冻保存即可立即获得特征系统。本文的假设是，通过适当的后勤和四室生物反应器技术，来自人类肝移植废物的细胞可以满足细胞治疗的需求，包括体外肝支持。
Initial results of the clinical use of primary porcine liver cells for extracorporeal liver support are being reviewed as the cell source is controversial. According to Eurotransplant data 20-25% of explanted donor livers are not transplanted, due to factors such as steatosis or cirrhosis. This number corresponds to the number of patients with acute liver failure who require bridging therapy to transplantation. Primary human liver cells from transplant discards can be isolated, purified and maintained in bioreactors and provide an alternative for cell-based extracorporeal liver support therapy. A four-compartment bioreactor enables recovery from preservation and isolation injury in a three-dimensional network of interwoven capillary membranes with integrated oxygenation, rendering the liver cells from these discarded donor organs viable for clinical utilization. Patient contact with additional animal-derived biomatrix and fetal calf serum can be avoided. The initiation of an in vitro cultivation phase allows cell stabilization, quality control, and immediate availability of a characterized system without cryopreservation. The hypothesis of this paper is that with appropriate logistics and four-compartment bioreactor technology, cells from human liver transplant discards can serve the demand for cell-based therapy, including extracorporeal liver support.