Incidence and characterization of Listeria monocytogenes from domestic and imported foods in Korea
Incidence and characterization of Listeria monocytogenes from domestic and imported foods in Korea
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DOI：
10.4315/0362-028x-63.2.186
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发表时间：
2000-02-01
影响因子：
2
通讯作者：
Kim, CM
中科院分区：
文献类型：
--
作者：
Baek, SY;Lim, SY;Kim, CM
在1993年至1997年期间，韩国共对1，537种国产和进口食品进行了单核细胞增生李斯特菌检测。L.使用美国农业部分离方法检测单核细胞增多症。孤立湖用从嗜酸乳杆菌溶血素O设计的hly 1和hly 2引物通过聚合酶链反应证实单核细胞增多症。总的来说，122个样品（7.9%）含有L。单核细胞增多症牛肉、猪肉、鸡肉、贝类、生乳、冷冻烟熏贻贝和冰淇淋的分离率分别为4.3%、19.1%、30.2%、1.2%、4.4%和6.1%。在巴氏杀菌牛奶、巴氏杀菌加工干酪、盐水鱼、干海产品或火腿中没有发现单核细胞增生李斯特菌。总体发病率低于其他国家先前研究报告的发病率。除鸡株以1/2a型为主外，其余均为1/2b型。血清分型结果可能提示存在食物或地理特异性L。单核细胞增生菌株。
A total of 1,537 domestic and imported food products were examined for the incidence of Listeria monocytogenes between 1993 and 1997 in Korea. L. monocytogenes was detected using the U.S. Department of Agriculture isolation method. Isolated L. monocytogenes was confirmed by polymerase chain reaction with hly1 and hly2 primers designed from the listeriolysin O. Overall, 122 samples (7.9%) contained L. monocytogenes. The rate of isolation was 4.3% for beef, 19.1% for pork, 30.2% for chicken, 1.2% for shellfish, 4.4% for raw milk, 4.4% for frozen smoked mussels, and 6.1% for ice cream. No L, monocytogenes was found in pasteurized milk pasteurized processed cheese, saltwater fish, dried seafoods, or ham. The overall incidence was lower than that reported in previous studies from other countries. Most isolates were serotype 1/2b except for chicken, in which serotype 1/2a was predominant. The serotyping results might imply the presence of food or geography-specific L. monocytogenes strains.