The biology of coral metamorphosis: Molecular responses of larvae to inducers of settlement and metamorphosis
The biology of coral metamorphosis: Molecular responses of larvae to inducers of settlement and metamorphosis
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DOI：
10.1016/j.ydbio.2011.02.010
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发表时间：
2011-05-15
影响因子：
2.7
通讯作者：
Ball, E. E.
中科院分区：
文献类型：
--
作者：
Grasso, L. C.;Negri, A. P.;Ball, E. E.
与许多其他刺胞动物一样，珊瑚经历从活动的浮浪幼虫到静止不动的息肉的变态。在一些海葵中，如线虫，这个过程是一个平稳的过渡，不需要外在刺激，但在许多珊瑚中，这个过程更加复杂，并且是由线索驱动的。为了更好地了解珊瑚变态背后的分子事件，用天然沉降诱导剂（结壳珊瑚藻碎片/提取物）或 LWamide 处理感受态幼虫，LWamide 绕过沉降阶段并直接驱动幼虫进入变态。使用具有> 8000个鹿角珊瑚unigenes的微阵列来跟踪这些处理后12小时内的基因表达变化，并通过原位杂交研究根据阵列实验选择的特定基因的表达模式。三种表达模式是常见的，一种是限制于搜索/定居阶段的中止表达模式，一种是对应于杯胚外胚层发育开始并在变态后继续的中止表达的第二阶段，以及一种后来的口服限制模式。 (C) 2011 Elsevier Inc. 保留所有权利。
Like many other cnidarians, corals undergo metamorphosis from a motile planula larva to a sedentary polyp. In some sea anemones such as Nematostella this process is a smooth transition requiring no extrinsic stimuli, but in many corals it is more complex and is cue-driven. To better understand the molecular events underlying coral metamorphosis, competent larvae were treated with either a natural inducer of settlement (crustose coralline algae chips/extract) or LWamide, which bypasses the settlement phase and drives larvae directly into metamorphosis. Microarrays featuring >8000 Acropora unigenes were used to follow gene expression changes during the 12 h period after these treatments, and the expression patterns of specific genes, selected on the basis of the array experiments, were investigated by in situ hybridization. Three patterns of expression were common an aboral pattern restricted to the searching/settlement phase, a second phase of aboral expression corresponding to the beginning of the development of the calicoblastic ectoderm and continuing after metamorphosis, and a later orally-restricted pattern. (C) 2011 Elsevier Inc. All rights reserved.