Evolutionary dynamics of rhodopsin type 2 opsins in vertebrates.
Evolutionary dynamics of rhodopsin type 2 opsins in vertebrates.
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脊椎动物视紫红质 2 型视蛋白的进化动力学。
DOI：
10.1093/molbev/msp217
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发表时间：
2010
影响因子：
10.7
通讯作者：
Tada,Takashi
中科院分区：
文献类型：
--
作者：
Yokoyama,Shozo;Tada,Takashi
在脊椎动物的5类视觉色素中，视紫红质2型（rhodopsin type 2, RH2）类群的基因重复事件最多。从北方旗鱼（Lepidopus fitchi）和棘鱼（Stenobrachius leucopsarus）中分别分离到3个完整的RH2视蛋白基因和1个无功能的RH2视蛋白基因。利用推导出的这些和其他具有代表性的脊椎动物RH2视蛋白基因的氨基酸序列，我们估计了在RH2视蛋白进化的不同阶段氨基酸取代的分化时间和进化速率。结果表明，旗鱼和剑鱼的RH2视蛋白的复制分别发生在~ 6000万年前和~ 3000万年前（Ma）。早期脊椎动物祖先中RH2视蛋白的进化速率为~ 0.25 × 10−9/位点/年，在真鱼谱系中为~ 1 × 10−9 ~ 3 × 10−9/位点/年，在腔棘鱼和四足动物谱系中为~ 0.3 × 10−9 ~ 0.5 × 10−9/位点/年。
Among the five groups of visual pigments in vertebrates, the rhodopsin type 2 (RH2) group shows the largest number of gene duplication events. We have isolated three intact and one nonfunctional RH2 opsin genes each from Northern lampfish (Stenobrachius leucopsarus) and scabbardfish (Lepidopus fitchi). Using the deduced amino acid sequences of these and other representative RH2 opsin genes in vertebrates, we have estimated the divergence times and evolutionary rates of amino acid substitution at various stages of RH2 opsin evolution. The results show that the duplications of the lampfish and scabbardfish RH2 opsins have occurred ∼60 and ∼30 million years ago (Ma), respectively. The evolutionary rates of RH2 opsins in the early vertebrate ancestors were ∼0.25 × 10−9/site/year, which increased to ∼1 × 10−9to 3 × 10−9/site/year in euteleost lineages and to ∼0.3 × 10−9to 0.5 × 10−9/site/year in coelacanth and tetrapods.