Anatomical, behavioural and physiological analyses of craniofacial development by cineradiographic imaging in marmosets
Anatomical, behavioural and physiological analyses of craniofacial development by cineradiographic imaging in marmosets
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DOI：
10.1111/joor.13323
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发表时间：
2022-03-31
影响因子：
2.9
通讯作者：
Lopes Zeredo，Jorge Luis
中科院分区：
文献类型：
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作者：
Oliveira，Luciana Rezende;Borges，Luana Siqueira;Lopes Zeredo，Jorge Luis
非人灵长类动物是在遗传学、生理学和行为学方面最接近人类的动物模型。特别是绒猴是生物医学research.ObjectiveAssessment的颅面生长和咀嚼功能的发展在普通绒猴（Callithrix jacchus），从出生到第四个月的生活，通过微创cinerradiographic imaging. Methods10个人进行了随访，从0到4个月的年龄颅面生长和咀嚼功能的cinerradiography评估。对于实验过程中，我们使用了微焦点X射线源装置和铍快速响应图像增强器。ResultsThe咀嚼周期的持续时间是稳定的年龄组。咀嚼非常软的Castella蛋糕或稍微硬一点的棉花糖不会改变时域中的咀嚼周期。另一方面，下颌张开运动的线性和角度测量显示，在颅面生长的后期阶段，有更大的运动趋势。定性分析表明，绒猴有一个小的偏好Castella棉花糖，他们最经常咬过的食物块咀嚼与他们的后牙，他们操纵的食物与他们的手，他们咀嚼的食物continuous.ConclusionWe观察到关键的发展事件在第一个3个月的生活在绒猴。在绒猴的电影放射成像可能提供有价值的信息颅面的形式和功能的基础和临床前研究模型。
BackgroundNon‐human primates are the closest animal models to humans regarding genetics, physiology and behaviour. Marmoset monkeys in particular are one of the most versatile species for biomedical research.ObjectiveTo assess the craniofacial growth and development of the masticatory function in the common marmoset (Callithrix jacchus), from birth to the fourth month of life through minimally invasive cineradiographic imaging.MethodsTen individuals were followed‐up from 0 to 4 months of age regarding craniofacial growth and masticatory function assessed by cineradiography. For the experimental procedure, we used a microfocal X‐ray source apparatus and a beryllium fast‐response image‐intensifier.ResultsThe duration of the masticatory cycles was stable across age groups. Chewing a very soft Castella cake or the slightly harder Marshmallow did not change the masticatory cycle in the time domain. On the other hand, linear and angular measurements of the jaw‐opening movement showed a tendency for bigger movements at the latter stages of craniofacial growth. Qualitative analysis showed that marmosets had a small preference for Castella over Marshmallow, that they most often bit off pieces of food to chew with their posterior teeth, that they manipulated the food with their hands, and that they chewed the food continuously.ConclusionWe observed critical developmental events during the first 3 months of life in marmosets. Cineradiographic imaging in marmosets may provide valuable information on craniofacial form and function for basic and preclinical research models.