Overexpression of ONAC054 Improves Drought Stress Tolerance and Grain Yield in Rice
Overexpression of ONAC054 Improves Drought Stress Tolerance and Grain Yield in Rice
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DOI：
10.3390/crops2040027
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发表时间：
2022-10
期刊：
影响因子：
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通讯作者：
Yasuhito Sakuraba;N. Paek
中科院分区：
文献类型：
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作者：
Yasuhito Sakuraba;N. Paek
干旱胁迫对植物生长和发育产生负面影响，从而降低植物生产力。因此，了解干旱胁迫反应的分子机制对作物改良至关重要。植物特异性NAM/ATAF 1，2/CUC 2（NAC）转录因子在干旱胁迫应答中发挥重要作用。在这里，我们表明，水稻（Oryza sativa）ONAC 054，膜结合NAC转录因子，参与干旱胁迫反应。我们发现，ONAC 054突变体是敏感的，而ONAC 054-过表达（ONAC 054-OX）的植物是耐受干旱胁迫。在干旱胁迫条件下，与脱落酸（阿坝）合成和信号转导相关的几个基因在onac 054突变体中下调，但在ONAC 054-OX植物中上调。在这些基因中，编码ABA诱导型bZIP转录因子的负责阿坝调节的转录因子1（TRAB 1）被ONAC 054直接激活。另一方面，ONAC 054的表达直接激活ABA-响应元件（ABRE）-结合因子（ABF）在ABA依赖的方式，表明ONAC 054作为ABA诱导的干旱胁迫耐受性的增强剂。此外，ONAC 054在水稻中的过表达极大地提高了干旱胁迫条件下的谷物产量，表明ONAC 054的过表达可以促进水稻和其他作物的干旱胁迫耐受性的提高。
Drought stress negatively affects plant growth and development, thus reducing plant productivity. Therefore, understanding the molecular mechanisms underlying drought stress responses is essential for crop improvement. The plant-specific NAM/ATAF1,2/CUC2 (NAC) transcription factors play important roles in the drought stress response. Here, we show that rice (Oryza sativa) ONAC054, a membrane-bound NAC transcription factor, is involved in the drought stress response. We found that onac054 mutants were sensitive, whereas ONAC054-overexpressing (ONAC054-OX) plants were tolerant to drought stress. Under drought stress conditions, several genes associated with abscisic acid (ABA) synthesis and signaling were downregulated in onac054 mutants but upregulated in ONAC054-OX plants. Among these genes, the TRANSCRIPTION FACTOR RESPONSIBLE FOR ABA REGULATION 1 (TRAB1), which encodes an ABA-inducible bZIP transcription factor, was directly activated by ONAC054. On the other hand, the expression of ONAC054 was directly activated by several ABA-responsive elements (ABRE)-binding factors (ABFs) in an ABA-dependent manner, indicating that ONAC054 acts as an enhancer of ABA-induced drought stress tolerance. Additionally, the overexpression of ONAC054 in rice greatly improved grain yield under drought stress conditions, indicating that the overexpression of ONAC054 could facilitate the improvement of drought stress tolerance in rice and other crops.